Priya Lohanathan Bharathi, Baskaran Rathinasamy, Elangovan Pitchai, Dhivya Velumani, Huang Chih-Yang, Padma Viswanadha Vijaya
Translational Research Laboratory, Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
Translational Research Laboratory, Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore 641046, Tamil Nadu, India; DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
Biomed Pharmacother. 2017 Mar;87:280-287. doi: 10.1016/j.biopha.2016.12.098. Epub 2017 Jan 4.
Persistence of cadmium (Cd) in the environment causes serious ecological problems. Tinospora cordifolia is a medicinal herb used in Ayurveda for treating various metabolic disorders and toxic conditions. The present study investigates the protective effect of T. cordifolia stem methanolic extract (TCME) on a heavy metal, Cd-induced cardiotoxicity in male Wistar rats. Male albino Wistar rats were divided into four groups (n=6). The animals after treatment for 28days with Cd and TCME were analysed for biochemical and histological changes in the serum and heart tissues. Cd induced lipid peroxidation and protein carbonylation was significantly reduced by TCME. TCME also reduced the histological alterations induced by Cd treatment in the heart tissues with diminished loss of myocardial fibers. Administration of TCME effectively prevented the altered levels of serum marker enzymes (creatine kinase and lactate dehydrogenase), antioxidants, such as superoxide dismutase, catalase, glutathione, glutathione peroxidase and glutathione-S-transferase, and glycoproteins contents such as hexose, hexoseamine, fucose, and sialic acid by Cd intoxication. TCME also offered protection against the change in levels of NaKATPase, MgATPase and CaATPase activities against Cd toxicity. The study suggests TCME as a potent cardioprotective agent against Cd induced toxicity.
镉（Cd）在环境中的持久性会引发严重的生态问题。心叶青牛胆是阿育吠陀医学中用于治疗各种代谢紊乱和中毒状况的一种草药。本研究调查了心叶青牛胆茎甲醇提取物（TCME）对重金属镉诱导的雄性Wistar大鼠心脏毒性的保护作用。雄性白化Wistar大鼠被分为四组（n = 6）。用镉和TCME处理28天后，对动物的血清和心脏组织进行生化和组织学变化分析。镉诱导的脂质过氧化和蛋白质羰基化被TCME显著降低。TCME还减少了镉处理诱导的心脏组织组织学改变，心肌纤维损失减少。给予TCME有效地预防了镉中毒导致的血清标志物酶（肌酸激酶和乳酸脱氢酶）、抗氧化剂（如超氧化物歧化酶、过氧化氢酶、谷胱甘肽、谷胱甘肽过氧化物酶和谷胱甘肽 - S - 转移酶）以及糖蛋白含量（如己糖、己糖胺、岩藻糖和唾液酸）水平的改变。TCME还对镉毒性引起的NaKATPase、MgATPase和CaATPase活性水平变化提供了保护。该研究表明TCME是一种有效的抗镉诱导毒性的心脏保护剂。
