马淋巴组织中B细胞分子的发育表达
Developmental expression of B cell molecules in equine lymphoid tissues.
作者信息
Prieto J M B, Tallmadge R L, Felippe M J B
机构信息
Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
出版信息
Vet Immunol Immunopathol. 2017 Jan;183:60-71. doi: 10.1016/j.vetimm.2016.12.004. Epub 2016 Dec 13.
Identification and classification of B cell subpopulations has been shown to be challenging and inconsistent among different species. Our study tested aspects of ontogeny, phenotype, tissue distribution, and function of equine CD5 B cells, which represented a greater proportion of B cells early in development and in the peritoneal cavity. CD5 and CD5 B cells differentially expressed B cell markers (CD2, CD21, IgM) measured using flow cytometry, but similar mRNA expression of signature genes (DGKA, FGL2, PAX5, IGHM, IL10) measured using quantitative RT-PCR. Sequencing lambda light chain segments revealed that CD5 B cells generated diverse immunoglobulin repertoires, and more frequently bound to fluorescence-labeled phosphorylcholine. This study shows developmental characteristics and tissue distribution of a newly described subpopulation of B cells in the horse.
B细胞亚群的鉴定和分类在不同物种间具有挑战性且不一致。我们的研究测试了马CD5 B细胞的个体发生、表型、组织分布和功能等方面，这些细胞在发育早期和腹腔中占B细胞的比例更大。使用流式细胞术测量发现，CD5和CD5 B细胞差异表达B细胞标志物（CD2、CD21、IgM），但使用定量RT-PCR测量时，特征基因（DGKA、FGL2、PAX5、IGHM、IL10）的mRNA表达相似。对λ轻链片段进行测序发现，CD5 B细胞产生了多样的免疫球蛋白库，并且更频繁地与荧光标记的磷酸胆碱结合。本研究展示了马中一个新描述的B细胞亚群的发育特征和组织分布。
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