一种新型季铵硅烷对牙本质蛋白酶活性的影响。

Effect of a novel quaternary ammonium silane on dentin protease activities.

作者信息

Umer D, Yiu C K Y, Burrow M F, Niu L-N, Tay F R

机构信息

Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Pokfulam, Hong Kong SAR, China.

出版信息

J Dent. 2017 Mar;58:19-27. doi: 10.1016/j.jdent.2017.01.001. Epub 2017 Jan 4.

DOI:10.1016/j.jdent.2017.01.001

PMID:28064012

Abstract

OBJECTIVES

Demineralized dentin collagen release C-terminal cross-linked telopeptide (ICTP) and C-terminal peptide (CTX) during degradation. The present study evaluated the effects of dentin pre-treatment with K21, a quaternary ammonium silane (QAS), on matrix metalloproteinase (MMP) and cathepsin K-mediated collagen degradation.

METHODS

Dentin beams were demineralized with 10% HPO for 24h. After baseline dry mass measurements, the beams were divided into 5 groups (N=10) according to protease inhibitors. The beams were pre-treated for 2min with 2% chlorhexidine (CHX), 2%, 5% or 10% QAS; no pre-treatment was performed for the control group. The beams were subsequently incubated in calcium- and zinc-containing medium for 3, 7 or 14days, after which changes in dry mass were measured and incubation media were examined for ICTP and CTX release. The MMP-2 and cathepsin K activities in QAS-treated dentin powder were also quantified using ELISA.

RESULTS

The two factors (disinfectants and time) had a significant effect on dry mass loss, ICTP and CTX release (p<0.001). The percentage of dry mass loss increased with time and was significantly lower in all experimental groups when compared to the control at 14days (p<0.001). Conversely, the rate of ICTP and CTX release was significantly lower in the experimental groups, compared to the uninhibited control at 7 and 14days (p<0.001). Dentinal MMP-2 and cathepsin K activities were significantly reduced after demineralized dentin was pre-treated with QAS.

CONCLUSION

The experimental QAS is a good inhibitor of MMP and cathepsin K activities in demineralized dentin.

CLINICAL SIGNIFICANCE

The newly developed antibacterial quaternary ammonium silane increases the resistance of dentin collagen to degradation by inhibiting endogenous matrix metalloproteinases and cysteine cathepsins. The quaternary ammonium silane cavity disinfectant is promising for use as a protease inhibitor to improve durability of resin-dentin bonds.

摘要

目的

脱矿牙本质胶原在降解过程中释放C端交联末肽（ICTP）和C端肽（CTX）。本研究评估了用季铵硅烷（QAS）K21预处理牙本质对基质金属蛋白酶（MMP）和组织蛋白酶K介导的胶原降解的影响。

方法

用10%HPO对牙本质梁进行脱矿处理24小时。在进行基线干质量测量后，根据蛋白酶抑制剂将梁分为5组（N = 10）。用2%氯己定（CHX）、2%、5%或10%的QAS对梁进行2分钟的预处理；对照组不进行预处理。随后将梁在含钙和锌的培养基中孵育3、7或14天，之后测量干质量的变化，并检测孵育培养基中ICTP和CTX的释放情况。还使用酶联免疫吸附测定法对QAS处理的牙本质粉末中的MMP-2和组织蛋白酶K活性进行定量。

结果

两个因素（消毒剂和时间）对干质量损失、ICTP和CTX释放有显著影响（p<0.001）。干质量损失百分比随时间增加，在14天时，所有实验组与对照组相比均显著降低（p<0.001）。相反，在7天和14天时，实验组中ICTP和CTX的释放率与未抑制的对照组相比显著降低（p<0.001）。用QAS预处理脱矿牙本质后，牙本质MMP-2和组织蛋白酶K活性显著降低。