Dafe Alireza, Etemadi Hossein, Zarredar Habib, Mahdavinia Gholam Reza
Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Science, Tabriz, Iran.
Young Researchers and Elite Club, Maragheh Branch, Islamic Azad University, Maragheh, Iran.
Int J Biol Macromol. 2017 Apr;97:299-307. doi: 10.1016/j.ijbiomac.2017.01.016. Epub 2017 Jan 5.
This study reports a novel carrier based on blends of carboxymethyl cellulose (CMC) and k-carrageenan (k-Carr) for probiotic colon delivery. Lactobacillus plantarum ATCC:13643 (L. plantarum) cells were encapsulated in CMC/k-Carr blends by extrusion method. k-Carrageenan was used as a coating agent to improve encapsulation of L. plantarum cells in carboxymethyl cellulose biopolymer. K-Carrageenan and carboxymethyl cellulose were ionically cross-linked with K and Ca ions, respectively. Optical and scanning electron microscopy obviously showed the random distribution of L. plantarum cells throughout the blend network. The viability of encapsulated cells in simulated gastric fluid (SGF) and bile salt solution were conducted. Results indicated that CMC/k-Carr blends could successfully protect L. plantarum cells against adverse conditions of the gastro-intestinal tract and bile salt solution. After sequential exposure to SGF for 2h almost complete death of free cells was observed. However, the number of surviving cells was 5.20 and 7.30 Log CFU/g for uncoated free CMC and CMC/k-Carr blends, respectively. Cumulatively the results of this research offer a suitable media to potentially deliver probiotics to colon site.
本研究报道了一种基于羧甲基纤维素（CMC）和κ-卡拉胶（κ-Carr）混合物的新型载体，用于益生菌的结肠递送。植物乳杆菌ATCC:13643（植物乳杆菌）细胞通过挤压法封装在CMC/κ-Carr混合物中。κ-卡拉胶用作包衣剂，以改善植物乳杆菌细胞在羧甲基纤维素生物聚合物中的封装。κ-卡拉胶和羧甲基纤维素分别与K离子和Ca离子进行离子交联。光学显微镜和扫描电子显微镜明显显示植物乳杆菌细胞在整个混合物网络中的随机分布。对封装细胞在模拟胃液（SGF）和胆盐溶液中的活力进行了检测。结果表明，CMC/κ-Carr混合物能够成功保护植物乳杆菌细胞免受胃肠道和胆盐溶液的不利条件影响。在连续暴露于SGF 2小时后，观察到游离细胞几乎完全死亡。然而，未包衣的游离CMC和CMC/κ-Carr混合物中存活细胞的数量分别为5.20和7.30 Log CFU/g。累积来看，本研究结果提供了一种合适的介质，有可能将益生菌递送至结肠部位。
