Gao Zhenzhen, Zhang Chao, Tian Weijun, Liu Kuanhui, Hou Ranran, Yue Chanjuan, Wu Yi, Wang Deyun, Liu Jiaguo, Hu Yuanliang, Yang Ying
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, PR China; Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, PR China.
Int J Biol Macromol. 2017 Apr;97:46-54. doi: 10.1016/j.ijbiomac.2017.01.013. Epub 2017 Jan 4.
Chinese angelica polysaccharides (CAP) and selenizing CAP (sCAP) were prepared and identified through FTIR and SEM observation. Their antioxidant activities in vitro and hepatoprotective effects in vivo were compared by free radical-scavenging tests or with CCl-induced hepatic injury model mice. The results showed that for DPPH radical, superoxide anion and hydroxyl radical, the scavenging capabilities of sCAP were significantly stronger than those of CAP In hepatic injury model mice, sCAP could significantly reduce ALT, AST and ALP contents and raised TP content in serum, significantly reduce MDA and ROS contents and raised SOD and T-AOC activities in liver homogenate in comparison with CAP; obviously relieve the pathological changes of liver and significantly inhibit the expressions of p-ERK, p-JNK and p-p38 protein as compared with those in model control group. These results indicate that selenylation modification can enhance the antioxidant and hepatoprotective actions of Chinese angelica polysaccharide. A action mechanism of sCAP is suppressing the protein expression of MAPK signaling pathway.
制备了当归多糖（CAP）和硒化当归多糖（sCAP），并通过傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）观察进行鉴定。通过自由基清除试验或利用四氯化碳诱导的肝损伤模型小鼠，比较了它们的体外抗氧化活性和体内肝脏保护作用。结果表明，对于二苯基苦味酰基自由基（DPPH）、超氧阴离子和羟基自由基，sCAP的清除能力明显强于CAP。在肝损伤模型小鼠中，与CAP相比，sCAP可显著降低血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）和碱性磷酸酶（ALP）含量，并提高总蛋白（TP）含量；显著降低肝脏匀浆中丙二醛（MDA）和活性氧（ROS）含量，并提高超氧化物歧化酶（SOD）和总抗氧化能力（T-AOC）活性；与模型对照组相比，明显减轻肝脏病理变化，并显著抑制磷酸化细胞外信号调节激酶（p-ERK）、磷酸化c-Jun氨基末端激酶（p-JNK）和磷酸化p38蛋白的表达。这些结果表明，硒化修饰可增强当归多糖的抗氧化和肝脏保护作用。sCAP的作用机制是抑制丝裂原活化蛋白激酶（MAPK）信号通路的蛋白表达。
