Rubtsov P M
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
Mol Biol (Mosk). 2016 Nov-Dec;50(6):935-943. doi: 10.7868/S0026898416060173.
Alternative splicing of pre-mRNA is one of the main mechanisms regulating gene expression that generates multiple transcripts from one gene. The review considers the specific role that intramolecular secondary structures arising through the interaction of distant complementary regions of introns play in regulating alternative splicing. Examples where aberrant splicing associated with hereditary disorders is corrected by altering the pre-mRNA secondary structures are described. The advances and prospects in whole transcriptome analysis of structured RNA regions and studies of their role in regulating alternative splicing are discussed.
前体mRNA的可变剪接是调控基因表达的主要机制之一，可从一个基因产生多个转录本。本文综述了内含子远距离互补区域相互作用产生的分子内二级结构在调控可变剪接中所起的特定作用。文中描述了通过改变前体mRNA二级结构来纠正与遗传性疾病相关的异常剪接的实例。讨论了结构化RNA区域全转录组分析及其在调控可变剪接中作用的研究进展与前景。
