[与ISIAH品系高血压大鼠相对肾脏重量和静息血压相关位点的差异表达基因]

[Differentially expressed genes in the locus associated with relative kidney weight and resting blood pressure in hypertensive rats of the ISIAH strain].

作者信息

Redina O E, Smolenskaya S E, Fedoseeva L A, Markel A L

机构信息

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090 Russia.

Novosibirsk State University, Novosibirsk, 630090 Russia.

出版信息

Mol Biol (Mosk). 2016 Nov-Dec;50(6):944-952. doi: 10.7868/S0026898416050141.

DOI:10.7868/S0026898416050141

PMID:28064310

Abstract

The comparative full-genome sequencing of transcriptomes of the renal cortex and medulla from hypertensive ISIAH rats and normotensive WAG rats revealed the differential expression of genes in the locus of chromosome 11 associated to the traits of resting blood pressure and relative kidney weight. Six differentially expressed genes (Kcne1, Rcan1, Mx1, Mx2, Tmprss2, and RGD1559516) were identified in the renal cortex, and three genes (Rcan1, Mx2, and Tmprss2) were identified in the renal medulla. An analysis of the functions of these genes pointed at the Rcan1 gene as the most relevant candidate gene associated with both the traits of resting blood pressure and relative kidney weight in ISIAH rats. The elevation of the transcription levels of the Mx1 and Mx2 genes in hypertensive ISIAH rats may represent an adaptation that contributes to the alleviation of inflammatory processes in the kidneys.

摘要

对高血压ISIAH大鼠和正常血压WAG大鼠肾皮质和髓质转录组进行的全基因组比较测序显示，11号染色体位点上与静息血压和相对肾重性状相关的基因存在差异表达。在肾皮质中鉴定出6个差异表达基因（Kcne1、Rcan1、Mx1、Mx2、Tmprss2和RGD1559516），在肾髓质中鉴定出3个基因（Rcan1、Mx2和Tmprss2）。对这些基因功能的分析表明，Rcan1基因是与ISIAH大鼠静息血压和相对肾重性状相关的最相关候选基因。高血压ISIAH大鼠中Mx1和Mx2基因转录水平的升高可能代表一种有助于减轻肾脏炎症过程的适应性变化。