Goolia Melissa, Vannucci Fabio, Yang Ming, Patnayak Devi, Babiuk Shawn, Nfon Charles K
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Manitoba, Canada (Goolia, Yang, Babiuk, Nfon).
Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN (Vannucci, Patnayak).
J Vet Diagn Invest. 2017 Mar;29(2):250-253. doi: 10.1177/1040638716683214. Epub 2017 Jan 8.
Senecavirus A (SVA; family Picornaviridae) is a nonenveloped, single-stranded RNA virus associated with idiopathic vesicular disease (IVD) in swine. SVA was detected in pigs with IVD in Brazil, United States, Canada, and China in 2015, triggering the need to develop and/or validate serologic assays for SVA. Our objective was to fully validate a previously developed competitive enzyme-linked immunosorbent assay (cELISA) as a screening test for antibodies to SVA. Additional objectives included the development and validation of a virus neutralization test (VNT) as a confirmatory test for SVA antibody detection, and the comparison of the cELISA, VNT, and an existing immunofluorescent antibody test (IFAT) for the detection of SVA antibodies in serial bleeds from SVA outbreaks. The diagnostic specificity and sensitivity were 98.2% (97.2-98.9%) and 96.9% (94.5-98.4%) for the cELISA, and 99.6% (99.0-99.9%) and 98.2% (95.8-99.4%) for the VNT, respectively. There was strong agreement among cELISA, VNT, and IFAT when compared based on kappa coefficient. Based on these performance characteristics, these tests are considered suitable for serologic detection of SVA in pigs.
A组赛内卡病毒（SVA；小RNA病毒科）是一种与猪特发性水疱病（IVD）相关的无包膜单链RNA病毒。2015年在巴西、美国、加拿大和中国的患有IVD的猪中检测到了SVA，这引发了开发和/或验证针对SVA的血清学检测方法的需求。我们的目标是全面验证先前开发的竞争酶联免疫吸附测定（cELISA）作为SVA抗体的筛查试验。其他目标包括开发和验证病毒中和试验（VNT）作为SVA抗体检测的确证试验，以及比较cELISA, VNT和现有的免疫荧光抗体试验（IFAT）在SVA疫情连续采血中检测SVA抗体的情况。cELISA的诊断特异性和敏感性分别为98.2%（97.2 - 98.9%）和96.9%（94.5 - 98.4%），VNT的诊断特异性和敏感性分别为99.6%（99.0 - 99.9%）和98.2%（95.8 - 99.4%）。基于kappa系数比较时，cELISA、VNT和IFAT之间有很强的一致性。基于这些性能特征，这些试验被认为适用于猪中SVA的血清学检测。
