Wu Xue-Liang, Xue Jun, Wang Li-Kun, Yang Dong-Dong, Qu Ming, Guo Fei, Sun Guang-Yuan, Han Lei, Yang Rui-Min
Department of Vascular Gland,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
Department of Ultrasound,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2016 Dec 20;38(6):696-701. doi: 10.3881/j.issn.1000-503X.2016.06.011.
Objective To explore the expressions of inhibitors of DNA binding-1 (Id-1) and matrix metalloproteinase-9 (MMP-9) in colorectal carcinoma tissues and its correlation with microvessel density (MVD). Methods The expressions of Id-1 and MMP-9 as well as CD34-labelled MVD in colorectal adenocarcinoma tissues (n=50) and normal adjacent tissues (n=50) were examined by immunohistochemistry. Results The positive expressions of Id-1 and MMP-9 were seen in 72.00% (36/50) and 78.00%(39/50) of colorectal adenocarcinoma tissues,which were significantly higher than those [24.00%(12/50) and 28.00% (14/50)] in normal adjacent tissues (P=0.000). The MVD value (17.22±2.08) in colorectal adenocarcinoma tissues was significantly higher than that (5.36±2.17) in normal adjacent tissues (P=0.000). The expressions of Id-1 and MMP-9 and MVD were significantly correlated with serosa invasion,TNM stage,carcinoembryonic antigen(+),lymph node metastasis,vascular invasion,and liver metastasis (all P<0.05) but not with the patient's age,gender,tumor size,and differentiation degree (all P>0.05). The MVD value with Id-1 and MMP-9 positive expression were significantly higher than those with Id-1 and MMP-9 negative expression (all P=0.000). The expression of Id-1 in colorectal adenocarcinoma tissues showed significantly positive correlation with that of MMP-9 (r=0.429,P=0.000). Cox multivariate analysis showed that Id-1 and MMP-9 expressions were independent prognostic factors for colorectal carcinoma. Conclusions The high expressions of Id-1 and MMP-9 have high correlations with the development and progression of colorectal adenocarcinoma and have positive correlation with MVD. Both of them may be involved in the microvascular generation and the invasion and hematogenous metastasis of colorectal carcinoma.
目的 探讨结直肠癌组织中DNA结合抑制因子-1（Id-1）和基质金属蛋白酶-9（MMP-9）的表达及其与微血管密度（MVD）的相关性。方法 采用免疫组织化学法检测50例结直肠腺癌组织及50例癌旁正常组织中Id-1、MMP-9的表达及CD34标记的MVD。结果 结直肠腺癌组织中Id-1和MMP-9的阳性表达率分别为72.00%（36/50）和78.00%（39/50），显著高于癌旁正常组织中的表达率[24.00%（12/50）和28.00%（14/50）]（P=0.000）。结直肠腺癌组织的MVD值（17.22±2.08）显著高于癌旁正常组织（5.36±2.17）（P=0.000）。Id-1、MMP-9的表达及MVD与浆膜侵犯、TNM分期、癌胚抗原（+）、淋巴结转移、血管侵犯及肝转移均显著相关（均P<0.05），但与患者年龄、性别、肿瘤大小及分化程度均无关（均P>0.05）。Id-1和MMP-9阳性表达组的MVD值显著高于Id-1和MMP-9阴性表达组（均P=0.000）。结直肠腺癌组织中Id-1的表达与MMP-9的表达呈显著正相关（r=0.429，P=0.000）。Cox多因素分析显示，Id-1和MMP-9的表达是结直肠癌的独立预后因素。结论 Id-1和MMP-9的高表达与结直肠腺癌的发生、发展密切相关，且与MVD呈正相关。二者可能均参与了结直肠癌的微血管生成及侵袭和血行转移过程。
