Bennett M, Meaders J, Ruvolo S, Burgess D
Boston College, Chestnut Hill, MA, United States.
Methods Cell Biol. 2017;137:1-13. doi: 10.1016/bs.mcb.2016.04.004. Epub 2016 Jun 28.
Sea urchins have long been used as a model organism to address questions in cell biology and development. Work utilizing this model has elucidated processes of fertilization, the description of centrosomes, the separation of chromosomes during mitosis, and numerous other landmark discoveries. They have been a favorite among researchers for a variety of reasons: Eggs are large and malleable, making them relatively easy to work with; the embryos have synchronized, rapid divisions; they are easy to microinject and manipulate; and their genome sequence is available. In this Chapter, we focus on methods used to study the membrane dynamics during cytokinesis using sea urchin embryos. The focus of this chapter is to provide methods for live-cell imaging of membrane domains in sea urchin embryos.
海胆长期以来一直被用作模式生物，以解决细胞生物学和发育方面的问题。利用该模式开展的研究阐明了受精过程、中心体的描述、有丝分裂期间染色体的分离以及许多其他具有里程碑意义的发现。由于多种原因，它们一直是研究人员的最爱：卵子大且具有可塑性，使其相对易于操作；胚胎具有同步、快速的分裂；它们易于显微注射和操作；并且其基因组序列是已知的。在本章中，我们重点介绍利用海胆胚胎研究胞质分裂过程中膜动力学的方法。本章的重点是提供海胆胚胎膜结构域活细胞成像的方法。
