Salem P, Denizot Y, Pitton C, Dulioust A, Bossant M J, Benveniste J, Thomas Y
Institut National de la Santé et de la Recherche Médicale, U 200 Université Paris-Sud, Clamart, France.
FEBS Lett. 1989 Oct 23;257(1):49-51. doi: 10.1016/0014-5793(89)81783-1.
Paf-acether (paf) is a phospholipid mediator of inflammation endowed with major immunoregulatory properties. The present study demonstrates that human thymus contains large amounts of paf, as well as paf precursors. In addition, isolated thymic cells produced paf under ionophore stimulation. Paf from thymus exhibited the same biological and physiochemical properties as synthetic paf. The purity and molecular structure of paf from thymus were further characterized by reverse-phase HPLC and gas chromatography with electron-capture detection. These findings may have important implications since thymus microenvironment is essential in the proper development of bone marrow progenitors committed to the T cell lineage into thymocytes capable of emigrating to the periphery as functional T lymphocytes.
血小板活化因子（PAF）是一种具有主要免疫调节特性的炎症磷脂介质。本研究表明，人类胸腺含有大量的PAF以及PAF前体。此外，分离的胸腺细胞在离子载体刺激下产生PAF。胸腺来源的PAF表现出与合成PAF相同的生物学和物理化学性质。通过反相高效液相色谱法和带电子捕获检测的气相色谱法进一步表征了胸腺来源PAF的纯度和分子结构。这些发现可能具有重要意义，因为胸腺微环境对于致力于T细胞谱系的骨髓祖细胞发育成能够作为功能性T淋巴细胞迁移到外周的胸腺细胞的正常发育至关重要。
