Eller M S, Sarkar S
Department of Anatomy and Cellular Biology, Tufts University, Boston, Massachusetts 02111.
Dev Biol. 1989 Nov;136(1):284-6. doi: 10.1016/0012-1606(89)90149-8.
The level of myosin light chain 3 (LC3) in vertebrate skeletal muscle is developmentally regulated in a tissue-specific manner. We have used the RNA-cDNA hybridization assay to quantitate LC3 mRNA levels at various stages of chick pectoralis muscle development in ovo. The LC3 mRNA was found significantly in breast muscle only on Day 16 in ovo and later, the level of mRNA ranging from about 30 to 32% of that present in adult tissue. These values are in good agreement with the corresponding levels of LC3 in embryonic muscle. These results do not support the earlier reports that the protein and mRNA for LC3 accumulate in a noncoordinate manner in embryonic pectoralis muscle and they suggest that LC3 synthesis in ovo is regulated primarily at the transcriptional level.
脊椎动物骨骼肌中肌球蛋白轻链3（LC3）的水平以组织特异性方式受到发育调控。我们利用RNA - cDNA杂交分析法对鸡胚胸肌发育各阶段的LC3 mRNA水平进行定量。仅在胚胎发育第16天及之后，才在胸肌中显著发现LC3 mRNA，其mRNA水平约为成年组织中水平的30%至32%。这些数值与胚胎肌肉中相应的LC3水平高度一致。这些结果不支持早期关于LC3蛋白和mRNA在胚胎胸肌中以非协调方式积累的报道，并且表明胚胎期LC3的合成主要在转录水平受到调控。
