成肌过程中肌球蛋白重链mRNA的定量分析。

Quantification of myosin heavy-chain mRNA during myogenesis.

作者信息

Robbins J, Heywood S M

出版信息

Eur J Biochem. 1978 Jan 16;82(2):601-8. doi: 10.1111/j.1432-1033.1978.tb12056.x.

DOI:10.1111/j.1432-1033.1978.tb12056.x

Abstract

Complementary DNA (cDNA) was synthesized from a 26-S poly(A)-containing RNA fraction consisting predominately of myosin mRNA, and used to quantitate the amounts of myosin mRNA during development. RNA . cDNAmyosin hybridizations were performed with RNA isolated from tertiary cultures of chick fibroblasts, and the proportion of myosin RNA determined. The levels of myosin RNA present in primary chick myoblast cultures undergoing development were also analyzed by RNA . cDNAmyosin hybridizations. Correlation of these values with the rate of myosin heavy chain synthesis indicates that myosin mRNA is subject to post-transcriptional or translational control.

摘要

互补DNA（cDNA）由主要包含肌球蛋白信使核糖核酸（mRNA）的26-S含聚腺苷酸（poly(A)）RNA组分合成，并用于定量发育过程中肌球蛋白mRNA的量。用从鸡成纤维细胞三级培养物中分离的RNA进行RNA-cDNA肌球蛋白杂交，并确定肌球蛋白RNA的比例。还通过RNA-cDNA肌球蛋白杂交分析了正在发育的原代鸡成肌细胞培养物中存在的肌球蛋白RNA水平。这些值与肌球蛋白重链合成速率的相关性表明，肌球蛋白mRNA受到转录后或翻译控制。