Hünkerler Zeynep, Köken Tülay, Koca Buğra, Kahraman Ahmet
Department of Clinical Biochemistry, School of Medicine, Afyon Kocatepe University, Afyon, Turkey.
Ther Apher Dial. 2017 Feb;21(1):38-42. doi: 10.1111/1744-9987.12504. Epub 2017 Jan 9.
Increased apoptotic cell death in uremic patients has been confirmed by a variety of studies. The present study aimed to investigate the effect of uremic toxins and duration of hemodialysis (HD) therapy on apoptosis by means of measuring serum caspase cleaved CK18 (CCCK-18) levels. Seventy chronic HD patients were recruited and divided into three groups with differing periods of HD, from 6 months to 10 years. Twelve healthy subjects served as controls. Serum CCCK-18 level was found significantly higher in HD patient groups (Group 2; 189 ± 71 IU/L, Group 3; 182 ± 65 IU/L, Group 4; 204 ± 111 IU/L) as compared to the control group (122 ± 20 U/L) (P < 0.05). When all hemodialysis patients considered together serum CCCK-18 showed positive correlation with serum uric acid and phosphorus (P < 0.05). In conclusion, our results suggest that apoptosis is enhanced in HD patients, phosphorus and uric acid might play a role in this increment, but duration of HD therapy has no effect on apoptosis.
多项研究已证实尿毒症患者凋亡性细胞死亡增加。本研究旨在通过检测血清半胱天冬酶裂解细胞角蛋白18（CCCK-18）水平，探讨尿毒症毒素及血液透析（HD）治疗时长对细胞凋亡的影响。招募了70例慢性HD患者，并将其分为HD时长不同的三组，时长从6个月至10年。12名健康受试者作为对照。与对照组（122±20 U/L）相比，HD患者组（第2组；189±71 IU/L，第3组；182±65 IU/L，第4组；204±111 IU/L）的血清CCCK-18水平显著更高（P<0.05）。当将所有血液透析患者综合考虑时，血清CCCK-18与血清尿酸和磷呈正相关（P<0.05）。总之，我们的结果表明HD患者的细胞凋亡增强，磷和尿酸可能在这种增加中起作用，但HD治疗时长对细胞凋亡无影响。
