Madhu Chakkere Shivamadhu, Manukumar Honnayakanahalli Marichenne Gowda, Basavaraju Puttalingaiah
Department of Biochemistry, Yuvaraja&#;s College, University of Mysore, India.
Pristine Laboratories, Kodigehalli Gate, Karnataka, India.
Acta Sci Pol Technol Aliment. 2014 Oct-Dec;13(4):375-383. doi: 10.17306/J.AFS.2014.4.4.
tudy describes antioxidant and anti-inflammatory properties of Sauropus androgynus leaf dialysed protein extract. Free radicals are implicated for many diseases including diabetes mellitus, arthritis, cancer, ageing etc. In the treatment of these diseases, antioxidant therapy has gained utmost importance. The main objective of the present study was to reveal the antioxidant and anti-inflammatory potentiality from S. androgynus leaf dialysed protein extract.
The antioxidant and anti-inflammatory properties of S. androgynus studied using different models viz. hydroxyl radical scavenging, DPPH radical scavenging, reducing power assay, superoxide radical scavenging activity by alkaline DMSO and phosphomolybdenum antioxidant assays and in vitro antiinflammatory activity by inhibition of protein denaturation, membrane stabilisation test models carried out.
Antioxidant activity was estimated in dose dependent manner showed hydroxyl radical (55.62%), DPPH (50%), reducing power (0.286 Abs), alkaline DMSO (72.51%) and phosphomolybdenum (0.198 Abs) activity high at 50 μg/ml concentration compared to standard curcumin (62.31%), curcumin (56.61%), tannic acid (0.54 Abs), curcumin (75.38%) and vitamin E (0.15 Abs) respectively. In-vitro anti-inflammatory activity by hypotonic induced model showed maximum protection (74.17%) compared to standard Acetylsalcylic acid (86.88%) at 100 μg/ml concentration and also in protein denaturation model protected protein denaturation maximum (83.60%) compared to standard Diclofenac (86.82%) at 100 μg/ml concentration respectively.
The antioxidant property usually studied related to the polyphenols and flavonoids present in the extract but present finding concluded that S. androgynus giving hint even potential proteins can also show responsible action effective against free radical mediated disease.
研究描述了守宫木叶片透析蛋白提取物的抗氧化和抗炎特性。自由基与许多疾病有关，包括糖尿病、关节炎、癌症、衰老等。在这些疾病的治疗中，抗氧化疗法变得至关重要。本研究的主要目的是揭示守宫木叶片透析蛋白提取物的抗氧化和抗炎潜力。
使用不同模型研究守宫木的抗氧化和抗炎特性，即羟自由基清除、DPPH自由基清除、还原能力测定、碱性二甲基亚砜超氧阴离子自由基清除活性和磷钼酸抗氧化测定，以及通过抑制蛋白质变性、膜稳定试验模型进行体外抗炎活性研究。
抗氧化活性以剂量依赖方式进行评估，在50μg/ml浓度下，羟自由基清除率（55.62%）、DPPH清除率（50%）、还原能力（0.286吸光度）、碱性二甲基亚砜清除率（72.51%）和磷钼酸清除率（0.198吸光度）均较高，分别与标准姜黄素（62.31%）、姜黄素（56.61%）、单宁酸（0.54吸光度）、姜黄素（75.38%）和维生素E（0.15吸光度）相比。在低渗诱导模型中，体外抗炎活性在100μg/ml浓度下显示出最大保护率（74.17%），与标准乙酰水杨酸（86.88%）相比，在蛋白质变性模型中，在100μg/ml浓度下对蛋白质变性的保护率也最高（83.60%），与标准双氯芬酸（86.82%）相比。
通常研究的抗氧化特性与提取物中存在的多酚和黄酮类化合物有关，但目前的研究结果表明，守宫木表明即使潜在的蛋白质也可能对自由基介导的疾病发挥有效作用。
