培养时间、接种量、温度、巴氏杀菌时间、山羊奶粉和乳清粉对植物乳杆菌LP69发酵乳中ACE抑制活性的影响。
Effect of incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder on ACE inhibitory activity in fermented milk by L. plantarum LP69.
作者信息
Shu Guowei, Yang Hui, Chen He, Zhang Qiuhong, Tian Yue
机构信息
College of Life Science and Engineering, Shaanxi University of Science and Technology, Xián, China.
出版信息
Acta Sci Pol Technol Aliment. 2015 Apr-Jun;14(2):107-116. doi: 10.17306/J.AFS.2015.2.12.
BACKGROUND
Angiotensin I converting enzyme (ACE) plays an important physiological role in regulating hypertension. Lactic acid bacteria are known to produce ACE inhibitory peptides which can lower hypertension during fermentation.
METHODS
The effect of incubation time (0~36 h), inoculum size (3, 4, 5, 6 and 7%, v/v), temperature (25, 30, 35, 40 and 45°C), sterilization time (5, 10, 15, 20 and 25 min), concentration of goat milk powder (8, 10, 12, 14 and 16%, w/v) and whey powder (0.5, 0.6, 0.7, 0.8 and 0.9%, w/v) on ACE inhibitory peptides fermented from goat milk by Lactobacillus plantarum LP69 was investigated using single factor experiment.
RESULTS
The optimal incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder in fermented milk by L. plantarum LP69 was 14 h, 3.0%, 35°C, 20 min, 14% and 0.70% for ACE inhibitory activity and 22 h, 3.0%, 40°C, 25 min, 16% and 0.60% for viable cell counts, respectively.
CONCLUSIONS
The incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder had a significant influence on ACE inhibitory activity in fermented milk by Lactobacillus plantarum LP69, the results are beneficial for further screening of main factors by using fractional factorial designs.
背景
血管紧张素I转换酶（ACE）在调节高血压中发挥重要的生理作用。已知乳酸菌在发酵过程中会产生可降低高血压的ACE抑制肽。
方法
采用单因素实验研究了培养时间（0~36小时）、接种量（3%、4%、5%、6%和7%，v/v）、温度（25℃、30℃、35℃、40℃和45℃）、杀菌时间（5分钟、10分钟、15分钟、20分钟和25分钟）、羊奶粉浓度（8%、10%、12%、14%和16%，w/v）以及乳清粉浓度（0.5%、0.6%、0.7%、0.8%和0.9%，w/v）对植物乳杆菌LP69发酵羊乳产生的ACE抑制肽的影响。
结果
植物乳杆菌LP69发酵乳中，对于ACE抑制活性，最佳培养时间、接种量、温度、巴氏杀菌时间、羊奶粉和乳清粉分别为14小时、3.0%、35℃、20分钟、14%和0.70%；对于活菌数，最佳培养时间、接种量、温度、巴氏杀菌时间、羊奶粉和乳清粉分别为22小时、3.0%、40℃、25分钟、16%和0.60%。
结论
培养时间、接种量、温度、巴氏杀菌时间、羊奶粉和乳清粉对植物乳杆菌LP69发酵乳中的ACE抑制活性有显著影响，这些结果有利于通过析因设计进一步筛选主要因素。
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