Carim Kellie J, Dysthe J Caleb, Young Michael K, McKelvey Kevin S, Schwartz Michael K
United States Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, Montana, United States of America.
PLoS One. 2017 Jan 9;12(1):e0169334. doi: 10.1371/journal.pone.0169334. eCollection 2017.
The Pacific lamprey (Entosphenus tridentatus) is an anadromous fish once abundant throughout coastal basins of western North America that has suffered dramatic declines in the last century due primarily to human activities. Here, we describe the development of an environmental DNA (eDNA) assay to detect Pacific lamprey in the Columbia River basin. The eDNA assay successfully amplified tissue derived DNA of Pacific lamprey collected from 12 locations throughout the Columbia River basin. The assay amplifies DNA from other Entosphenus species found outside of the Columbia River basin, but is species-specific within this basin. As a result, the assay presented here may be useful for detecting Entosphenus spp. in geographic range beyond the Columbia River Basin. The assay did not amplify tissue or synthetically derived DNA of 14 commonly sympatric non-target species, including lampreys of the genus Lampetra, which are morphologically similar to Pacific lamprey in the freshwater larval stage.
太平洋七鳃鳗（Entosphenus tridentatus）是一种溯河洄游鱼类，曾在北美西部沿海流域大量存在，但在上个世纪主要由于人类活动而数量急剧下降。在此，我们描述了一种环境DNA（eDNA）检测方法的开发，用于检测哥伦比亚河流域的太平洋七鳃鳗。该eDNA检测方法成功扩增了从哥伦比亚河流域12个地点采集的太平洋七鳃鳗的组织衍生DNA。该检测方法能扩增出哥伦比亚河流域以外发现的其他七鳃鳗属物种的DNA，但在该流域内具有物种特异性。因此，本文介绍的检测方法可能有助于在哥伦比亚河流域以外的地理范围内检测七鳃鳗属物种。该检测方法未扩增14种常见的同域非目标物种的组织或合成衍生DNA，包括在淡水幼体阶段形态与太平洋七鳃鳗相似的八目鳗属七鳃鳗。
