Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
Ultrason Sonochem. 2017 May;36:42-49. doi: 10.1016/j.ultsonch.2016.10.030. Epub 2016 Nov 1.
Separation and detection of residual drug in biological fluids has directly related to human health in term of their beneficial or side effects. In biological fluid samples (complex matrix which containing drug at very low level) conduction of preliminary efficient technique as good choice for pre-concentration and clean-up of real sample before their instrumental analysis is highly recommended. In this result technique, based on nano-structure material which poses higher available surface area and larger member of reactive sites led to significant improvement in characteristic performance of analytical method. This paper focused on the synthesis and application of novel nano-sorbent for pre-concentration and extraction of atorvastatin (AT) from different biological fluids. Influence of various variables including sorbent dosage, eluent volume and sonication time on present method response was studied and optimized by central composite design under response surface methodology and accordingly an acceptable calibration curves over wide linear ranges (0.3-2000μgL) with high coefficient of determination higher than 0.999 strongly confirm high applicability of present method for quantification of analyte while limit of detection and quantification in plasma sample were 0.0608 and 0.2033μg L, respectively.
在生物体液样本（含有药物的复杂基质，其药物浓度非常低）中，在进行仪器分析之前，采用初步高效技术对其进行预浓缩和净化，这与药物的有益或副作用直接相关，与人类健康息息相关。在这种技术中，基于纳米结构材料，其具有更高的可用表面积和更多的反应位点，这导致分析方法的特征性能得到了显著改善。本文主要研究了一种新型纳米吸附剂的合成及其在阿托伐他汀（AT）从不同生物体液中预浓缩和提取的应用。通过中心复合设计，在响应面法的指导下，研究并优化了各种变量（包括吸附剂用量、洗脱体积和超声时间）对本方法响应的影响。结果表明，本方法在较宽的线性范围内（0.3-2000μg/L）具有较高的线性相关系数（r>0.999），表明本方法具有良好的适用性，可用于分析物的定量检测；而在血浆样品中的检测限和定量限分别为 0.0608 和 0.2033μg/L。
