Dong Xiao-Long, Liu Tai-Hang, Wang Wei, Pan Cai-Xia, Du Guo-Yu, Wu Yun-Fei, Adur Malavika, Zhang Min-Juan, Pan Min-Hui, Lu Cheng
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
Biochem Biophys Res Commun. 2017 Feb 5;483(2):855-859. doi: 10.1016/j.bbrc.2017.01.017. Epub 2017 Jan 6.
Our previous study has identified a gene, BmREEPa, which affects BmNPV invasion in silkworm cells. In this study, we interfered with BmREEPa in silkworm larvae through transgenic technology and screened BmREEPa-RNAi silkworm strains (RP). We found the mortality in RP was lower than that in Dazao, when silkworm larvae were infected with BmNPV via oral and injection routes. And the expression level of VP39 was lower in RP than in Dazao in the group infected via injection. In the oral infection group, VP39 expression level showed significant reduction at 48 h post-infection. These results revealed that the anti-BmNPV activity was enhanced in RP, and this enhancement probably presents itself during secondary infection via BVs.
我们之前的研究已经鉴定出一个基因BmREEPa，它影响家蚕细胞中BmNPV的入侵。在本研究中，我们通过转基因技术在家蚕幼虫中干扰BmREEPa，并筛选出BmREEPa-RNAi家蚕品系（RP）。我们发现，当通过口服和注射途径感染BmNPV时，RP的死亡率低于大造。在注射感染组中，RP中VP39的表达水平低于大造。在口服感染组中，感染后48小时VP39表达水平显著降低。这些结果表明，RP的抗BmNPV活性增强，这种增强可能在通过芽生病毒进行二次感染时表现出来。
