Zhu Baojian, Tang Lin, Yu Yingying, Yu Huimin, Wang Lei, Qian Cen, Wei Guoqing, Liu Chaoliang
College of Life Sciences, Anhui Agricultural University, Hefei 230036, PR China.
College of Life Sciences, Anhui Agricultural University, Hefei 230036, PR China.
Gen Comp Endocrinol. 2017 May 15;246:372-381. doi: 10.1016/j.ygcen.2017.01.013. Epub 2017 Jan 6.
The hepatopancreas of crustaceans plays an important role in lipid and carbohydrate metabolism, digestion of food, and biogenesis. In this study, the hepatopancreas transcriptome from the red crayfish Procambarus clarkii was characterized for the first time using high-throughput sequencing, producing approximately 41.4 million reads were obtained. After de novo assembly, 57,363 unigenes with an average length of 725bp were identified, Gene Ontology analysis categorized 22,580 as being involved in biological processes, among which metabolic process and cellular process groups were the most highly enriched. A total of 8034 unigenes were assigned to 223 metabolic pathways following mapping against the Kyoto encyclopedia of genes and genomes (KEGG) database. Ecdysteroid receptor (EcR)-mediated signaling pathways were investigated using digital gene expression (DGE) analysis following RNA interference targeting the EcR. A total of 529 differentially expressed genes (DEGs) were identified, including 322 downregulated and 207 upregulated unigenes. Of these, 445 (84.12%) were annotated successfully by alignment with known sequences, many of which were related to catalytic activity and binding functional categories. Using KEGG enrichment analysis, 183 DEGs were clustered into 78 pathways, and six significantly enriched pathways were predicted. The expression patterns of candidate genes identified by real-time PCR were consistent with the DGE results.
甲壳类动物的肝胰腺在脂质和碳水化合物代谢、食物消化以及生物合成中发挥着重要作用。在本研究中，首次利用高通量测序对克氏原螯虾的肝胰腺转录组进行了表征，获得了约4140万个 reads。经过从头组装，鉴定出57363个单基因，平均长度为725bp，基因本体分析将22580个归类为参与生物过程，其中代谢过程和细胞过程组的富集程度最高。在与京都基因与基因组百科全书（KEGG）数据库比对后，共有8034个单基因被分配到223条代谢途径中。在针对蜕皮激素受体（EcR）进行RNA干扰后，利用数字基因表达（DGE）分析研究了EcR介导的信号通路。共鉴定出529个差异表达基因（DEG），包括322个下调的和207个上调的单基因。其中，445个（84.12%）通过与已知序列比对成功注释，其中许多与催化活性和结合功能类别相关。利用KEGG富集分析，183个DEG被聚类到78条途径中，并预测出6条显著富集的途径。通过实时PCR鉴定的候选基因的表达模式与DGE结果一致。
