Li Shengjie, Shen Li, Sun Lianjie, Xu Jiao, Jin Ping, Chen Liming, Ma Fei
Laboratory for Comparative Genomics and Bioinformatics, Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, China.
The Key Laboratory of Developmental Genes and Human Disease, College of Life Science, Nanjing Normal University, Nanjing 210046, China.
Dev Comp Immunol. 2017 May;70:80-87. doi: 10.1016/j.dci.2017.01.008. Epub 2017 Jan 7.
Drosophila have served as a model for research on innate immunity for decades. However, knowledge of the post-transcriptional regulation of immune gene expression by microRNAs (miRNAs) remains rudimentary. In the present study, using small RNA-seq and bioinformatics analysis, we identified 67 differentially expressed miRNAs in Drosophila infected with Escherichia coli compared to injured flies at three time-points. Furthermore, we found that 21 of these miRNAs were potentially involved in the regulation of Imd pathway-related genes. Strikingly, based on UAS-miRNAs line screening and Dual-luciferase assay, we identified that miR-9a and miR-981 could both negatively regulate Drosophila antibacterial defenses and decrease the level of the antibacterial peptide, Diptericin. Taken together, these data support the involvement of miRNAs in the regulation of the Drosophila Imd pathway.
几十年来，果蝇一直是先天免疫研究的模型。然而，关于微小RNA（miRNA）对免疫基因表达的转录后调控的知识仍然很基础。在本研究中，我们使用小RNA测序和生物信息学分析，在三个时间点上，鉴定出与受伤果蝇相比，感染大肠杆菌的果蝇中有67个差异表达的miRNA。此外，我们发现其中21个miRNA可能参与Imd通路相关基因的调控。引人注目的是，基于UAS-miRNA品系筛选和双荧光素酶测定，我们确定miR-9a和miR-981都可以负向调节果蝇的抗菌防御，并降低抗菌肽双翅菌素的水平。综上所述，这些数据支持miRNA参与果蝇Imd通路的调控。
