Koussoroplis Apostolos-Manuel, Schwarzenberger Anke, Wacker Alexander
Theoretical Aquatic Ecology an Ecophysiology Group, Institute for Biochemistry and Biology, University of Potsdam, 14469 Potsdam, Germany
Theoretical Aquatic Ecology an Ecophysiology Group, Institute for Biochemistry and Biology, University of Potsdam, 14469 Potsdam, Germany.
Biol Open. 2017 Feb 15;6(2):210-216. doi: 10.1242/bio.022046.
We studied the short- (12 h) and long-term (144 h) response of lipases to quality shifts in diets consisting of different mixtures of the green alga with the cyanobacterium , two species with contrasting lipid compositions. The lipase/esterase activity in both the gut and the body tissues had fast responses to the diet shift and increased with higher dietary contributions of When screening the genome for TAG lipases, we discovered a large gene-family expansion of these enzymes. We used a subset of eight genes for mRNA expression analyses and distinguished between influences of time and diet on the observed gene expression patterns. We identified five diet-responsive lipases of which three showed a sophisticated short- and long-term pattern of expression in response to small changes in food-quality. Furthermore, the gene expression of one of the lipases was strongly correlated to lipase/esterase activity in the gut suggesting its potentially major role in digestion. These findings demonstrate that the lipid-related enzymatic machinery of is finely tuned to diet and might constitute an important mechanism of physiological adaptation in nutritionally complex environments.
我们研究了脂肪酶对由绿藻与蓝细菌不同混合物组成的饮食质量变化的短期（12小时）和长期（144小时）反应，这两种藻类的脂质组成截然不同。肠道和身体组织中的脂肪酶/酯酶活性对饮食变化反应迅速，并且随着[某种藻类]在饮食中所占比例的增加而升高。在筛选[某种生物]基因组中的三酰甘油脂肪酶时，我们发现这些酶的基因家族大幅扩张。我们选取了八个基因的子集进行mRNA表达分析，并区分时间和饮食对观察到的基因表达模式的影响。我们鉴定出五种对饮食有反应的脂肪酶，其中三种对食物质量的微小变化呈现出复杂的短期和长期表达模式。此外，其中一种脂肪酶的基因表达与肠道中的脂肪酶/酯酶活性密切相关，表明其在消化过程中可能发挥主要作用。这些发现表明，[某种生物]与脂质相关的酶机制能很好地适应饮食，可能是在营养复杂环境中生理适应的重要机制。
