Qu Jialin, Yan Xinjia, Li Chunyan, Wen Jing, Lu Chongning, Ren Jungang, Peng Ying, Song Shaojiang
School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Rd., Shenyang 110016, China.
Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, 222 Zhongshan Rd., Dalian 116011, China.
J Chromatogr Sci. 2017 Apr 1;55(4):451-458. doi: 10.1093/chromsci/bmw203.
A multi-component quantification fingerprint based on high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry technique has been established for the comparative analysis of raw and ripe fruits of Forsythia suspensa originated from different provinces. Eighteen bioactive constituents including three phenylethanoid glycosides derivatives, six phenolic acids, three flavonoids, four phenylpropanoids, one fatty acid and one terpenoid were identified and quantified. Total contents of phenylethanoid glycosides, phenylpropanoids and flavonoids from raw samples were found much higher than those from ripe samples, while total content of phenolic acids showed a contrary tendency. Moreover, the anti-microbial activities were comparatively assayed for the first time using five different bacterial strains. Results revealed a positive relationship between contents of total phenolic and anti-microbial activity. The results obtained in the present study may provide useful information for future utilization of F. suspensa.
基于高效液相色谱-电喷雾电离串联质谱技术建立了一种多成分定量指纹图谱，用于比较分析不同省份连翘生果和熟果。鉴定并定量了18种生物活性成分，包括3种苯乙醇苷衍生物、6种酚酸、3种黄酮类化合物、4种苯丙素类化合物、1种脂肪酸和1种萜类化合物。发现生样品中苯乙醇苷、苯丙素类化合物和黄酮类化合物的总含量远高于熟样品，而酚酸的总含量则呈现相反的趋势。此外，首次使用5种不同的细菌菌株比较测定了抗菌活性。结果表明总酚含量与抗菌活性之间呈正相关。本研究所得结果可为连翘的未来利用提供有用信息。
