Tan Ying, Feng Qing, Sun Xin, Xue Min, Jiang Ning, Deng Xinliang
Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Dec 28;41(12):1305-1311. doi: 10.11817/j.issn.1672-7347.2016.12.009.
To investigate the reversal effect of methylseleninic acid on cisplatin (DDP)-resistant ovarian cancer cells and the underlying mechanisms. Methods: SKOV3/DDP cells were incubated with cisplatin at different concentrations for 48 h, then the proliferation rate of SKOV3/DDP cells was detected by MTT assays, and the expression of β-catenin in SKOV3/DDP cells was examined by Western blot. The inhibitory effect of methyl-seleninic acid (MSA) combined with DDP at different concentrations on SKOV3/DDP cells was assayed by MTT method. Western blot was used to detect the expression of β-catenin protein in the cells. Results: The inhibitory rate for proliferation in DDP-treated SKOV3/DDP cells with different concentrations is lower than that in the SKOV3 cells (P<0.05); β-catenin expression in SKOV3/DDP cells was significantly higher than that in the SKOV3 cells (P<0.05). The inhibitory rate for proliferation in SKOV3/DDP cells with different concentrations of MSA was increased with the increase in concentration (P<0.05). The inhibitory rate for proliferation in SKOV3/DDP cells with 2 or 6 μmol/L MSA plus cisplatin was lower than that in cisplatin alone group (P<0.05). β-catenin expression in SKOV3 /DDP cells with 2 or 6 μmol/L MSA plus cisplatin was higher than that in the cisplatin alone group (P<0.05). Conclusion: MSA can reverse cisplatin resistance on SKOV3 / DDP cells, which may be related to the decrease in β-catenin expression.
探讨甲基亚硒酸对顺铂（DDP）耐药卵巢癌细胞的逆转作用及其潜在机制。方法：将SKOV3/DDP细胞与不同浓度的顺铂孵育48小时，然后通过MTT法检测SKOV3/DDP细胞的增殖率，并通过蛋白质印迹法检测SKOV3/DDP细胞中β-连环蛋白的表达。采用MTT法检测不同浓度的甲基亚硒酸（MSA）联合DDP对SKOV3/DDP细胞的抑制作用。用蛋白质印迹法检测细胞中β-连环蛋白的表达。结果：不同浓度顺铂处理的SKOV3/DDP细胞的增殖抑制率低于SKOV3细胞（P<0.05）；SKOV3/DDP细胞中β-连环蛋白的表达明显高于SKOV3细胞（P<0.05）。不同浓度MSA处理的SKOV3/DDP细胞的增殖抑制率随浓度增加而升高（P<0.05）。2或6μmol/L MSA加顺铂处理的SKOV3/DDP细胞的增殖抑制率低于顺铂单独处理组（P<0.05）。2或6μmol/L MSA加顺铂处理的SKOV3/DDP细胞中β-连环蛋白的表达高于顺铂单独处理组（P<0.05）。结论：MSA可逆转SKOV3/DDP细胞对顺铂的耐药性，这可能与β-连环蛋白表达降低有关。
