Nenadić Marija, Soković Marina, Glamočlija Jasmina, Ćirić Ana, Perić-Mataruga Vesna, Ilijin Larisa, Tešević Vele, Todosijević Marina, Vujisić Ljubodrag, Vesović Nikola, Ćurčić Srećko
Institute of Zoology, University of Belgrade - Faculty of Biology, Belgrade, Serbia.
Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
Appl Microbiol Biotechnol. 2017 Feb;101(3):977-985. doi: 10.1007/s00253-016-8082-7. Epub 2017 Jan 9.
Recently, various insect secretions have been tested as possible antimicrobial agents. In beetles, these secretions are essentially products of various exocrine glands, of which particular emphasis is on pygidial glands that are common for the suborder Adephaga. The antimicrobial potential of the pygidial gland secretion isolated from the adults of Calosoma sycophanta against human pathogens has been tested and compared with the potential of other carabid species, particularly within the tribe Carabini. The antimicrobial assay includes a microdilution method which was applied in order to determine the minimal inhibitory, minimal fungicidal and minimal bactericidal concentrations. It has been tested the effect of the secretion against eight strains of fungal and eight strains of bacterial species. The secretion sample of the tested carabid species has shown statistically significant antifungal effect against all strains of treated micromycetes, the highest in comparison with previously tested carabids (Carabus spp., Laemostenus punctatus and Abax parallelepipedus). Aspergillus versicolor proved to be the most sensitive micromycete, while the remaining seven fungal strains have shown the same level of sensibility. In comparison with commercial mycotics ketoconazole and bifonazole, applied as positive controls, the tested secretion showed much higher antifungal activity for all fungal strains. Antibacterial effect has been manifested only against one bacterial strain (Escherichia coli), contrary to other previously studied carabid species. These observations might have a significant impact on the ecological domain and possible purpose in biomedical studies and applications in the future. Additionally, morpho-histology of the pygidial glands of C. sycophanta is investigated and discussed.
最近，人们对各种昆虫分泌物作为潜在抗菌剂进行了测试。在甲虫中，这些分泌物基本上是各种外分泌腺的产物，其中特别强调的是在肉食亚目昆虫中常见的臀腺。已经对从步甲Calosoma sycophanta成虫中分离出的臀腺分泌物对人类病原体的抗菌潜力进行了测试，并与其他步甲科物种，特别是在步甲族中的抗菌潜力进行了比较。抗菌试验包括一种微量稀释法，用于确定最小抑菌浓度、最小杀真菌浓度和最小杀菌浓度。已经测试了该分泌物对8种真菌菌株和8种细菌菌株的作用。受试步甲物种的分泌物样本对所有受试的微真菌菌株均显示出具有统计学意义的抗真菌作用，与之前测试的步甲（Carabus spp.、Laemostenus punctatus和Abax parallelepipedus）相比，其抗真菌作用最强。杂色曲霉被证明是最敏感的微真菌，而其余7种真菌菌株显示出相同的敏感性水平。与作为阳性对照使用的市售抗真菌药酮康唑和联苯苄唑相比，受试分泌物对所有真菌菌株均表现出更高的抗真菌活性。与其他先前研究的步甲物种相反，其抗菌作用仅在一种细菌菌株（大肠杆菌）上表现出来。这些观察结果可能会对生态领域产生重大影响，并在未来的生物医学研究和应用中具有潜在用途。此外，还对Calosoma sycophanta臀腺的形态组织学进行了研究和讨论。
