Levacher C, Picon L
Laboratoire de Physiologie du Développment, Unité associée au Centre National de la Recherche Scientifique 307, Université Paris, France.
Horm Metab Res. 1989 Oct;21(10):537-41. doi: 10.1055/s-2007-1009282.
In the rat, hyperthyroidism induced from birth has been shown to increase both adipose cell recruitment and de novo lipogenesis at 6 weeks of age. Since preadipose cells are included into the stromavascular fraction (SVF) of adipose tissue, fatty acid synthetizing enzyme activities were estimated separately in adipocytes and SVF cells of adipose tissue of 6 week-old hyperthyroid rats. Fatty acid synthetase (FAS), malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activities were generally increased in adipocytes and in SVF cells when compared to age-matched controls. Among the enzymes studied, ME activity displayed the highest stimulation in SVF cells and was much more stimulated in these cells than in adipocytes (214% and 73% increase, respectively, above control values, P less than 0.001). These results show that, in vivo, SVF cells and adipocytes can respond differently to an hormonal stimulation and raise the question of the role of ME in the adipose conversion.
在大鼠中，已表明从出生起就诱导的甲状腺功能亢进会在6周龄时增加脂肪细胞募集和从头脂肪生成。由于前脂肪细胞包含在脂肪组织的血管基质部分（SVF）中，因此分别估计了6周龄甲状腺功能亢进大鼠脂肪组织的脂肪细胞和SVF细胞中的脂肪酸合成酶活性。与年龄匹配的对照组相比，脂肪细胞和SVF细胞中的脂肪酸合成酶（FAS）、苹果酸酶（ME）、葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）活性通常会增加。在所研究的酶中，ME活性在SVF细胞中表现出最高的刺激，并且在这些细胞中的刺激程度远高于脂肪细胞（分别比对照值增加214%和73%，P小于0.001）。这些结果表明，在体内，SVF细胞和脂肪细胞对激素刺激的反应可能不同，并提出了ME在脂肪转化中的作用问题。
