Schmidt P, Peter H H, Kalden J R, Avenarius H J, Bodenstein H
Klin Wochenschr. 1978 Oct 1;56(19):953-62. doi: 10.1007/BF01480149.
Blood lymphocytes from 13 untreated acute leukemia patients, 3 pre-leukemias 3 immunoblastic lymphadenopathias and one infectious mononucleosis showed significantly lower spontaneous (SCMC) and antibody-dependent cellular cytotoxicity (ADCC) against 51Cr-labeled allogeneic melanoma cells of the IGR3 cell line than effector lymphocytes from 20 age- and sex matched control persons. While control lymphocytes exhibited the highest cytotoxic activity after depletion of mononuclear phagocytes (Fraction FFF), followed by the "Ficoll" purified Fraction F and defibrinated whole blood, the reverse was true for acute leukemias: here, the highest cytotoxicity was found in whole blood followed by the lymphocyte fractions F and FFF. Comparatively high cytotoxicity was found with two leukemia patients who had received blood transfusions the day before testing. During the course of an acute erythroleukemia chemotherapy drastically reduced SCMC and ADCC activities. A therapeutical splenectomy, on the other hand, did not affect cellular cytotoxicity in the case of a hairy cell leukemia. The angioimmunoblastic lymphadenopathies showed strikingly high percentages of EA- and EAC-rosettes forming cells and showed a marked increase of SCMC and ADCC activities after elimination of mononuclear phagocytes from the effector cell population.
13例未经治疗的急性白血病患者、3例白血病前期患者、3例免疫母细胞性淋巴结病患者及1例传染性单核细胞增多症患者的血液淋巴细胞，与20名年龄和性别匹配的对照者的效应淋巴细胞相比，对IGR3细胞系的51Cr标记同种异体黑色素瘤细胞的自发细胞介导细胞毒性（SCMC）和抗体依赖性细胞毒性（ADCC）显著降低。对照淋巴细胞在单核吞噬细胞耗竭后（FFF组分）表现出最高的细胞毒性活性，其次是“Ficoll”纯化的F组分和去纤维蛋白全血，而急性白血病患者则相反：这里，全血中细胞毒性最高，其次是淋巴细胞组分F和FFF。在两名检测前一天接受输血的白血病患者中发现了相对较高的细胞毒性。在急性红白血病化疗过程中，SCMC和ADCC活性急剧降低。另一方面，治疗性脾切除术对毛细胞白血病患者的细胞毒性没有影响。血管免疫母细胞性淋巴结病显示出形成EA和EAC玫瑰花结细胞的百分比极高，并且在从效应细胞群体中去除单核吞噬细胞后，SCMC和ADCC活性显著增加。
