Isogai Masamichi, Kamata Yukie, Ando Syunpei, Kamata Misaki, Shirakawa Asuka, Sekine Ken-Taro, Yoshikawa Nobuyuki
Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka 020-8550, Iwate, Japan.
Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka 020-8550, Iwate, Japan.
Virology. 2017 Mar;503:6-11. doi: 10.1016/j.virol.2017.01.002. Epub 2017 Jan 7.
Gentian ovary ring-spot virus (GORV) infected gentian plants by pollination with GORV-infected gentian pollen grains, but the virus was not horizontally transmitted to gentian plants by transfer of pollen from GORV-infected Nicotiana benthamiana plants. However, N. benthamiana plants were infected with the virus by pollination with infected gentian pollen as well as by pollination with infected N. benthamiana pollen. When infected gentian pollen grains were placed on N. benthamiana stigmas, germinating pollen tubes penetrated into the stigmas and the styles (stigma-style). Virus infection occurred during penetration of the stigma-style, and the virus subsequently spread systemically to the mother plant. On the other hand, most infected N. benthamiana pollen grains failed to germinate on gentian stigmas, and virus infections were not detected in the stigma-style.
龙胆卵巢环斑病毒（GORV）通过用感染了GORV的龙胆花粉粒授粉来感染龙胆植物，但该病毒不会通过将来自感染GORV的本氏烟草植物的花粉转移而水平传播到龙胆植物上。然而，本氏烟草植物通过用感染的龙胆花粉授粉以及用感染的本氏烟草花粉授粉而感染该病毒。当将感染的龙胆花粉粒放置在本氏烟草柱头上时，萌发的花粉管穿透柱头和花柱（柱头-花柱）。病毒感染在柱头-花柱的穿透过程中发生，随后病毒全身扩散到母本植物。另一方面，大多数感染的本氏烟草花粉粒在龙胆柱头上未能萌发，并且在柱头-花柱中未检测到病毒感染。
