Patra Digambara, Mishra A
Department of Chemistry, Indian Institute of Technology Madras, Chennai - 600 036, India, India.
Anal Bioanal Chem. 2002 Jul;373(4-5):304-309. doi: 10.1007/s00216-002-1330-y. Epub 2014 Apr 13.
Extending the two-dimensional synchronous fluorescence scan to a three-dimensional total synchronous fluorescence scan (TSFS) spectral measurement gives the total synchronous fluorescence characteristics of a multifluorophoric sample at various possible wavelength intervals (Δλ), which could help to characterize multifluorophoric systems better. TSFS spectra of petroleum products such as diesel, kerosene, petrol, engine oil etc., available in the Indian market, are reported. Fluorescence in these samples is due to the presence of polycyclic aromatic hydrocarbons (PAHs) of various ring sizes. The TSFS contour plot profiles of the neat samples measured at right-angle geometry is a result of various energy-degrading photophysical processes such as inner filter effect, light attenuation, resonance energy transfer, collisional quenching etc. TSFS plots make it easy to obtain the optimized Δλ of an unknown sample of analytical interest. TSFS and the excitation-emission matrix (EEM) techniques are similar, but the contour profiles generated are different. The response of the TSFS contour profiles to dilution is different from that in the EEM contour profiles. Thus, TSFS can provide an alternative way of presenting the fluorescence response of concentrated multifluorophoric samples.
将二维同步荧光扫描扩展为三维全同步荧光扫描（TSFS）光谱测量，可以得到多荧光团样品在各种可能波长间隔（Δλ）下的全同步荧光特性，这有助于更好地表征多荧光团体系。报道了印度市场上柴油、煤油、汽油、机油等石油产品的TSFS光谱。这些样品中的荧光是由于存在各种环大小的多环芳烃（PAHs）。在直角几何条件下测量纯净样品的TSFS等高线图轮廓是各种能量降解光物理过程的结果，如内滤效应、光衰减、共振能量转移、碰撞猝灭等。TSFS图便于获得感兴趣分析未知样品的最佳Δλ。TSFS和激发-发射矩阵（EEM）技术相似，但生成的等高线轮廓不同。TSFS等高线轮廓对稀释的响应与EEM等高线轮廓不同。因此，TSFS可以提供一种呈现浓缩多荧光团样品荧光响应的替代方法。
