He Jinying, Tuo Ya, Yan Wenjie, Yang Jing
Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, China.
Reproductive Medicine Center, Inner Mongolia People's Hospital, Hohhot, China.
Cytotechnology. 2017 Feb;69(1):117-122. doi: 10.1007/s10616-016-0043-6. Epub 2017 Jan 10.
Bacterial surface layers (S-layers) are cell envelope structures ubiquitously found in gram-negative and gram-positive bacteria, including Lactobacillus. S-layers play a role in the determination and maintenance of cell shape as virulence factors, mediate cell adhesion, and regulate immature dendritic and T cells. In this study, we sought to understand the involvement of MAPK serine/threonine kinases in alterations in Endometrial epithelial cells (ESC) growth induced by Lactobacillus crispatus (L. crispatus) slpA, an S-layer protein. We applied various concentrations of L. crispatus to cultured ESCs and observed growth and changes in the phosphorylation status of ERK1/2, JNK, and p38. Similar experiments were conducted using L. crispatus lacking and overexpressing slpA. We found that ESC growth was altered by slpA primarily via ERK1/2. Our findings suggest that L. crispatus slpA promotes ESC growth mainly through an ERK1/2-dependent pathway.
细菌表面层（S层）是在革兰氏阴性菌和革兰氏阳性菌（包括乳酸杆菌）中普遍存在的细胞包膜结构。S层作为毒力因子在细胞形状的确定和维持中发挥作用，介导细胞粘附，并调节未成熟树突状细胞和T细胞。在本研究中，我们试图了解丝裂原活化蛋白激酶（MAPK）丝氨酸/苏氨酸激酶在卷曲乳杆菌（L. crispatus）的S层蛋白slpA诱导的子宫内膜上皮细胞（ESC）生长改变中的作用。我们将不同浓度的卷曲乳杆菌应用于培养的ESC，并观察ERK1/2、JNK和p38的磷酸化状态的生长和变化。使用缺乏和过表达slpA的卷曲乳杆菌进行了类似的实验。我们发现，slpA主要通过ERK1/2改变ESC的生长。我们的研究结果表明，卷曲乳杆菌slpA主要通过ERK1/2依赖性途径促进ESC生长。
