Islam Md F, Adhikari Suraj, Paik Man-Jeong, Lee Wonjae
College of Pharmacy, Chosun University, Gwangju, 501-759, Korea.
College of Pharmacy, Sunchon National University, Sunchon, Jeonnam, 540-950, Korea.
Arch Pharm Res. 2017 Mar;40(3):350-355. doi: 10.1007/s12272-016-0883-9. Epub 2017 Jan 10.
The liquid chromatographic enantiomer separation of various chiral acids as 1-naphthylamides was performed using several chiral stationary phases (CSPs). The CSPs used in this study were six covalently bonded and four coated type CSPs derived from amylose and cellulose derivatives as chiral selectors. The degree of enantioseparation is affected by the structure of chiral acids and the CSPs used, which have different chiral selectors and types of immobilization. For the enantiomer resolution of chiral acids as 1-naphthylamide derivatives, the performance of the coated type Lux Cellulose-1 was superior to those of the other CSPs, except for 2-aryloxypropionic acid derivatives. Owing to the strong ultraviolet absorbance of the 1-naphthyl group, the convenient analytical method developed and validated in this study could be expected to be very useful for the enantiomer separation of various chiral acids as 1-naphthylamide derivatives using polysaccharide-derived CSPs.
使用几种手性固定相（CSP）对各种手性酸的1-萘酰胺进行了液相色谱对映体分离。本研究中使用的CSP是六种共价键合型和四种涂覆型CSP，它们源自直链淀粉和纤维素衍生物作为手性选择剂。对映体分离程度受手性酸结构和所用CSP的影响，这些CSP具有不同的手性选择剂和固定化类型。对于作为1-萘酰胺衍生物的手性酸的对映体拆分，除2-芳氧基丙酸衍生物外，涂覆型Lux Cellulose-1的性能优于其他CSP。由于1-萘基具有很强的紫外吸收，预计本研究开发并验证的便捷分析方法对于使用多糖衍生CSP对各种手性酸的1-萘酰胺衍生物进行对映体分离非常有用。
