Kökoğlu Çağrı, Delen Emre, Arslantaş Ali, Arslantaş Didem, Kökoğlu Burcu, Özbek Zühtü, Uslu Sema, Akıncı Ahmet Tolgay
Department of Neurosurgery, Eskişehir Yunus Emre State Hospital, Eskişehir-Turkey.
Ulus Travma Acil Cerrahi Derg. 2016 Nov;22(6):521-525. doi: 10.5505/tjtes.2016.37571.
This experimental study was an investigation of the efficacy of erythropoietin and tadalafil in rats with induced spinal cord injury (SCI).
Thirty-five Sprague Dawley rats were distributed into 5 groups. First group was used for normal biochemical values. Spinal cord injury was induced in 4 remaining groups with clip compression technique after laminectomy process to T10 vertebra. Second group was designated solvent group and received 1 cc physiological serum after injury. Third group was medicated with intraperitoneal 2000 u/kg single dose erythropoietin after injury. Orogastric 2 mg/kg single dose tadalafil was administered to fourth group after injury. Fifth group did not receive any treatment and was used for biochemical values with injury. All subjects were sacrificed 48 hours after application. Malondialdehyde (MDA) and total antioxidant capacity (TAOC) values were evaluated using blood and tissue samples.
Lowest serum and tissue MDA values were found in group with erythropoietin intake. While highest serum TAOC values of all groups were seen in tadalafil group, highest tissue TAOC values were observed in group given erythropoietin.
It was concluded that by decreasing oxidative stress, tadalafil and erythropoietin can inhibit secondary damage in SCI.
本实验研究旨在探讨促红细胞生成素和他达拉非对诱导性脊髓损伤（SCI）大鼠的疗效。
将35只Sprague Dawley大鼠分为5组。第一组用于获取正常生化值。对其余4组大鼠在T10椎体椎板切除术后采用夹压技术诱导脊髓损伤。第二组为溶剂组，损伤后给予1毫升生理血清。第三组在损伤后腹腔注射单剂量2000 u/kg促红细胞生成素。第四组在损伤后经口胃管给予单剂量2 mg/kg他达拉非。第五组不接受任何治疗，用于获取损伤后的生化值。所有受试动物在用药后48小时处死。使用血液和组织样本评估丙二醛（MDA）和总抗氧化能力（TAOC）值。
促红细胞生成素摄入组的血清和组织MDA值最低。虽然所有组中血清TAOC值最高的是他达拉非组，但组织TAOC值最高的是促红细胞生成素给药组。
得出的结论是，他达拉非和促红细胞生成素可通过降低氧化应激来抑制脊髓损伤中的继发性损伤。
