白细胞介素-1β与肿瘤坏死因子-α在3T3细胞产生大鼠多形核白细胞趋化因子过程中的协同作用。

Synergism between interleukin-1 beta and tumor necrosis factor-alpha in production by 3T3 cells of a chemotactic factor for rat polymorphonuclear leukocytes.

作者信息

Nakagawa H, Miyai H, Hirata M, Watanabe K, Onuma I

机构信息

Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.

出版信息

Inflammation. 1989 Oct;13(5):553-9. doi: 10.1007/BF00916761.

DOI:10.1007/BF00916761

PMID:2807520

Abstract

Recombinant human interleukin-1 beta (IL-1) and tumor necrosis factor-alpha (TNF) synergistically stimulated BALB/c 3T3 cells to produce a chemotactic factor for rat polymorphonuclear leukocytes (PMNs), whereas an addition of 10(-11)-10(-8) M IL-1 or TNF alone to the cell culture resulted in a slight increase in the production of chemotactic factor. The partially purified factor was not a chemokinetic but chemotactic factor for PMNs when analyzed by checkerboard analysis. The partially purified factor was trypsin sensitive and heat stable; its isoelectric point was 8.5-10, and its molecular weight was about 10 kDa as estimated by gel filtration. These results suggest that fibroblasts may participate in PMN migration to the inflammatory site where both IL-1 and TNF are released by activated inflammatory cells, including macrophages.

摘要

重组人白细胞介素-1β（IL-1）和肿瘤坏死因子-α（TNF）协同刺激BALB/c 3T3细胞产生一种对大鼠多形核白细胞（PMN）具有趋化作用的因子，而在细胞培养中单独添加10^(-11)-10^(-8) M的IL-1或TNF只会使趋化因子的产生略有增加。通过棋盘分析可知，部分纯化的因子对PMN不是化学促动因子而是趋化因子。部分纯化的因子对胰蛋白酶敏感且热稳定；其等电点为8.5 - 10，通过凝胶过滤估计其分子量约为10 kDa。这些结果表明，成纤维细胞可能参与PMN向炎症部位的迁移，在该部位，包括巨噬细胞在内的活化炎症细胞会释放IL-1和TNF。