Irvine L, Travers M, Simpson K, Lowe G D, Courtney J M
Bioengineering Unit, University of Strathclyde, Glasgow, U.K.
Int J Artif Organs. 1989 Aug;12(8):502-4.
The advantages of multiparameter assessment in the evaluation of the blood compatibility of biomaterials underline the importance of investigating possible relevant parameters. In this respect, a study has been made of granulocyte elastase to establish the influence of haemodialysis membranes on the release of this serine proteinase. Plasma levels of granulocyte elastase were determined as a complex with its natural inhibiter, alpha 1-proteinase. This elastase - alpha 1 proteinase (E- alpha 1 Pi) inhibitor complex was measured by a highly sensitive enzyme-linked immunoassay. The membranes evaluated were Cuprophan (15-11, Travenol) and polysulphone (F40, Fresenius). Samples were taken from patients undergoing maintenance haemodialysis, before the start of dialysis, after 15 and 90 minutes and again at the end of dialysis (4 h). This investigation clearly demonstrates the different response of the dialysers, and on correcting for surface area, the end-dialysis level of E-alpha 1 Pi remained significantly greater for the Cuprophan membrane. The results support the view that the release of granulocyte elastase is a relevant parameter for inclusion in membrane compatibility assessment.
多参数评估在生物材料血液相容性评价中的优势凸显了研究可能相关参数的重要性。在这方面，已对粒细胞弹性蛋白酶展开研究，以确定血液透析膜对这种丝氨酸蛋白酶释放的影响。粒细胞弹性蛋白酶的血浆水平是作为与其天然抑制剂α1-蛋白酶的复合物来测定的。这种弹性蛋白酶-α1蛋白酶（E-α1Pi）抑制剂复合物通过高灵敏度的酶联免疫测定法进行检测。所评估的膜为铜仿膜（15-11，特拉文诺尔）和聚砜膜（F40，费森尤斯）。样本取自接受维持性血液透析的患者，在透析开始前、透析15分钟和90分钟后以及透析结束时（4小时）采集。该研究清楚地表明了透析器的不同反应，并且在对表面积进行校正后，铜仿膜的E-α1Pi透析结束时水平仍显著更高。结果支持这样一种观点，即粒细胞弹性蛋白酶的释放是纳入膜相容性评估的一个相关参数。
