Velho G, Reach G
Service de Diabétologie, Hôtel-Dieu, Paris, France.
Int J Artif Organs. 1989 Aug;12(8):539-43.
Bioreactors for cell culture, in which hollow fibers are sealed into a protective jacket, cells are seeded in the fibers' outer surface and a culture medium circulates through the fibers, have been proposed as a bioartificial pancreas. We used a needle-type glucose sensor to study the kinetics of glucose transfer across the membrane of one such device. The glucose transfer was found to be dependent on the flow rate of the circulating medium, which suggests the involvement of an ultrafiltration flux across the membrane. The glucose concentration was heterogeneous within the cell compartment. This heterogeneity, and the delay in transmission of changes in glucose concentration from the circulating medium to the cell compartment, can be ascribed to the large volume of the compartment. The design of these bioreactors should therefore be modified, in order to meet the requirements of glucose transfer kinetics of a bioartificial pancreas.
用于细胞培养的生物反应器，其中中空纤维被密封在一个保护套中，细胞接种在纤维的外表面，培养基在纤维中循环，已被提议作为一种生物人工胰腺。我们使用针型葡萄糖传感器来研究葡萄糖通过其中一种装置的膜的转运动力学。发现葡萄糖转运取决于循环介质的流速，这表明存在跨膜超滤通量。细胞隔室内的葡萄糖浓度是不均匀的。这种不均匀性，以及葡萄糖浓度变化从循环介质传递到细胞隔室的延迟，可归因于隔室的大体积。因此，这些生物反应器的设计应加以改进，以满足生物人工胰腺葡萄糖转运动力学的要求。
