Gulseren Duygu, Süzük-Yıldız Serap, Çelebi Bekir, Kılıç Selçuk
a Department of Dermatology , Ankara Polatlı State Hospital , Ankara , Turkey and.
b Department of Microbiology Reference Laboratory , Public Health Institution of Turkey , Ankara , Turkey.
Cutan Ocul Toxicol. 2017 Sep;36(3):289-293. doi: 10.1080/15569527.2017.1281288. Epub 2017 Feb 2.
Anthrax, caused by the bacterium Bacillus anthracis, is one of the oldest documented infectious diseases in both livestock and humans. We aimed to evaluate clinical findings and risk factors of patients with cutaneous anthrax infection and report anti-lethal factor (LF) IgG and anti-protective antigen (PA) IgG titers in the serologic diagnosis of disease.
In this study, serum samples of 18 cutaneous anthrax patients were collected and anti-LF IgG and anti-PA IgG titers were measured by enzyme-linked immunosorbent assay (ELISA).
Twelve (67%) males and 6 (33%) females, with a mean age of 36.06 ± 16.58 years were included in the study. Risk factors identified in the patient population studied were slaughtering (28%), flaying (56%), chopping meat (67%), burying diseased animal corpses (17%) and milking (6%) livestock. Black eschar formation (94%), pruritus (78%) and painful lymphadenopathy (61%) were first three common clinical signs and symptoms, respectively. Fourteen (78%) patients produced a positive IgG response against PA, 11 (61%) patients produced against LF. Three (17%) patients had no response to either antigen.
A detailed history of contact with sick animals or animal products along with clinical findings should be taken at the first step for the diagnosis of cutaneous anthrax infection. Serologic detection of anti-LF IgG and anti-PA IgG with ELISA may be useful auxillary method for establishing the diagnosis.
由炭疽芽孢杆菌引起的炭疽病是有文献记载的最古老的人畜共患传染病之一。我们旨在评估皮肤炭疽感染患者的临床症状和危险因素，并报告疾病血清学诊断中抗致死因子（LF）IgG和抗保护性抗原（PA）IgG滴度。
本研究收集了18例皮肤炭疽患者的血清样本，采用酶联免疫吸附测定（ELISA）法检测抗LF IgG和抗PA IgG滴度。
研究纳入12名（67%）男性和6名（33%）女性，平均年龄为36.06±16.58岁。在研究的患者群体中确定的危险因素包括屠宰（28%）、剥皮（56%）、剁肉（67%）、掩埋病死动物尸体（17%）和挤奶（6%）家畜。黑色焦痂形成（94%）、瘙痒（78%）和疼痛性淋巴结病（61%）分别是前三个常见的临床症状。14名（78%）患者对PA产生阳性IgG反应，11名（61%）患者对LF产生反应。3名（17%）患者对两种抗原均无反应。
诊断皮肤炭疽感染时，第一步应详细询问与患病动物或动物产品接触的病史以及临床症状。用ELISA法进行抗LF IgG和抗PA IgG的血清学检测可能是有助于确诊的辅助方法。
