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黑曲霉E-1中由同一单基因xlsIV（xlnD）编码的功能性β-木糖苷酶的定位

Localization of functional β-xylosidases, encoded by the same single gene, xlsIV (xlnD), from Aspergillus niger E-1.

作者信息

Inoue Kotomi, Takahashi Yui, Obara Ken, Murakami Shuichiro

机构信息

a Department of Agricultural Chemistry , School of Agriculture, Meiji University , Kawasaki , Japan.

出版信息

Biosci Biotechnol Biochem. 2017 Mar;81(3):621-624. doi: 10.1080/09168451.2016.1268040. Epub 2017 Jan 12.

DOI:10.1080/09168451.2016.1268040
PMID:28077024
Abstract

Cell wall-associated β-xylosidase was isolated from Aspergillus niger E-1 and identified as XlsIV, corresponding to the extracellular enzyme XlnD reported previously. xlsIV was transcribed only in the early cultivation period. Cell wall-associated enzyme activity gradually decreased, but extracellular activity increased as the strain grew. These results indicate that XlsIV (XlnD) was secreted into culture after localizing at cell wall.

摘要

从黑曲霉E-1中分离出细胞壁相关的β-木糖苷酶，并鉴定为XlsIV，它与先前报道的细胞外酶XlnD相对应。XlsIV仅在培养初期转录。随着菌株的生长，细胞壁相关酶活性逐渐降低，但细胞外活性增加。这些结果表明，XlsIV（XlnD）在定位于细胞壁后被分泌到培养基中。

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