Koshiguchi Manami, Komazaki Hitoshi, Hirai Shizuka, Egashira Yukari
a Laboratory of Food and Nutrition, Graduate School of Horticulture , Chiba University , Chiba , Japan.
Biosci Biotechnol Biochem. 2017 May;81(5):966-971. doi: 10.1080/09168451.2016.1274636. Epub 2017 Jan 12.
Ferulic acid (FA) is a phenol compound found in plants that has anti-inflammatory properties. Indoleamine 2, 3-dioxygenase (IDO) is a tryptophan catabolic enzyme induced in immune cells, including glial cells, during inflammation. Enhanced IDO expression leads to reduced tryptophan levels and increased levels of toxic metabolites, including quinolinic acid. Therefore, inhibition of IDO expression may be effective in suppressing progression of neurodegenerative diseases. In this study, we examined the effect of FA in microglial cells on IDO expression levels and related inflammatory signal molecules. FA suppressed LPS-induced IDO mRNA expression and also suppressed nuclear translocation of NF-κB and phosphorylation of p38 MAPK. However, FA did not affect the production of LPS-induced inflammatory mediators and phosphorylation of JNK. Our results indicate that FA suppresses LPS-induced IDO mRNA expression, which may be mediated by inhibition of the NF-κB and p38 MAPK pathways in microglial cells.
阿魏酸（FA）是一种存在于植物中的具有抗炎特性的酚类化合物。吲哚胺2,3-双加氧酶（IDO）是一种在炎症期间在包括神经胶质细胞在内的免疫细胞中诱导产生的色氨酸分解代谢酶。IDO表达增强会导致色氨酸水平降低以及包括喹啉酸在内的有毒代谢物水平升高。因此，抑制IDO表达可能对抑制神经退行性疾病的进展有效。在本研究中，我们研究了FA对小胶质细胞中IDO表达水平及相关炎症信号分子的影响。FA抑制了LPS诱导的IDO mRNA表达，还抑制了NF-κB的核转位和p38 MAPK的磷酸化。然而，FA并不影响LPS诱导的炎症介质的产生以及JNK的磷酸化。我们的结果表明，FA抑制LPS诱导的IDO mRNA表达，这可能是通过抑制小胶质细胞中的NF-κB和p38 MAPK途径介导的。
