Bilal Fatima, Pérès Michaël, Le Faouder Pauline, Dupuy Aude, Bertrand-Michel Justine, Andrieu-Abadie Nathalie, Levade Thierry, Badran Bassam, Daher Ahmad, Ségui Bruno
INSERM UMR 1037, CRCT, 31037, Toulouse, France.
Equipe Labellisée Ligue Contre Le Cancer, 31037, Toulouse, France.
Methods Mol Biol. 2017;1557:213-217. doi: 10.1007/978-1-4939-6780-3_20.
Sphingolipids are sphingoid base-containing lipids, among which some metabolites behave as bioactive molecules in various biological processes, including cell death. Whereas ceramide is now viewed as an anti-oncometabolite, leading to cancer cell death, CD95L-induced apoptosis is associated with sphingolipid changes, which likely contribute to caspase-dependent signaling pathway activation. Here, we describe Liquid Chromatography-high resolution mass spectrometry method (LC-HRMS) to analyze sphingolipid metabolism changes triggered by CD95L.
鞘脂是含鞘氨醇碱基的脂质，其中一些代谢产物在包括细胞死亡在内的各种生物过程中表现为生物活性分子。虽然神经酰胺现在被视为一种抗代谢物，可导致癌细胞死亡，但CD95L诱导的细胞凋亡与鞘脂变化有关，这可能有助于半胱天冬酶依赖性信号通路的激活。在此，我们描述了液相色谱-高分辨率质谱法（LC-HRMS），用于分析由CD95L触发的鞘脂代谢变化。
