Department of Histology (Tissue Engineering Group), University of Granada, Granada, Spain.
Department of Biomedical Sciences, Texas A&M University Baylor College of Dentistry, Dallas, TX, USA.
J Tissue Eng Regen Med. 2018 Jan;12(1):204-216. doi: 10.1002/term.2397. Epub 2017 Jul 30.
Regeneration of the pulp-dentin complex with stem cells is a potential alternative to conventional root canal treatments. Human dental pulp stem cells (hDPSCs) have been extensively studied because of their ability to proliferate and differentiate into mineralized dental and non-dental tissues. Here we combined hDPSCs with two types of injectable poly-l-lactic acid (PLLA) microsphere with a nanofibrous or smooth surface to form bioactive injectable aggregates, and examined their ability to promote pulp regeneration in the root canal in an in vivo model. We investigated the biocompatibility, biosafety and odontogenic potential of fibrous (F-BIM) and smooth bioactive injectable microspheres (S-BIM) in vitro and in vivo. Our results demonstrated that PLLA microspheres and hDPSCs were able to form bioactive injectable aggregates that promoted dentin regeneration in both in vitro and in vivo models. Our results suggest that F-BIM and S-BIM may induce dentinogenesis upon in vivo grafting, and propose that the potential usefulness of the microsphere-hDPSC aggregates described here should be evaluated in clinical settings. Copyright © 2017 John Wiley & Sons, Ltd.
牙髓-牙本质复合体的干细胞再生是一种替代传统根管治疗的潜在方法。由于人牙髓干细胞（hDPSCs）具有增殖和分化为矿化牙和非牙组织的能力，因此它们被广泛研究。在这里，我们将 hDPSCs 与两种类型的可注射聚 L-乳酸（PLLA）微球结合，这些微球具有纳米纤维或光滑表面，形成具有生物活性的可注射聚集体，并在体内模型中研究了它们促进根管内牙髓再生的能力。我们研究了纤维状（F-BIM）和光滑生物活性可注射微球（S-BIM）在体外和体内的生物相容性、生物安全性和牙原性潜力。我们的结果表明，PLLA 微球和 hDPSCs 能够形成生物活性可注射聚集体，促进体外和体内模型中的牙本质再生。我们的结果表明，F-BIM 和 S-BIM 可能在体内移植时诱导牙本质形成，并提出应在临床环境中评估本文所述微球-hDPSC 聚集体的潜在用途。版权所有 © 2017 约翰威立父子公司
