Andreasen A, Holm I E, Geneser F A
Department of Rheumatology, Viborg City Hospital, Denmark.
Histochem J. 1989 Aug;21(8):481-9. doi: 10.1007/BF01845798.
The distribution of acetylcholinesterase (AChE) was analysed in the hippocampal region of the rabbit, employing computerized optical densitometry (COD). AChE was demonstrated histochemically according to a modification of the copper thiocholine method, and computerized analysis was performed on a selected section with the use of a graphic operating processor, resulting in images with pixel values ranging between 0 and 255, measuring 512 X 512 pixels. Examples of the densitometric analysis include presentation of pixel values in single points, in profiles through selected areas and in slicing procedures. The results of the densitometric analysis basically agreed with the subjective visual impression of different staining intensities in the sections and corresponding photomicrographs. However, the densitometric analysis provided an objective and more exact expression of the relative AChE content of the different subfields and layers of the hippocampal region. In particular, zones with the same enzyme content as well as zones differing only minimally in activity can be recognized easily and unequivocally. In view of this, the promising future uses of COD are considered briefly.
采用计算机光学密度测定法（COD）分析了家兔海马区乙酰胆碱酯酶（AChE）的分布情况。根据硫代胆碱铜方法的改进对AChE进行组织化学染色，并使用图形操作处理器对选定切片进行计算机分析，得到像素值在0至255之间、尺寸为512×512像素的图像。密度分析的示例包括单点像素值呈现、选定区域的轮廓呈现以及切片程序。密度分析结果与切片及相应显微照片中不同染色强度的主观视觉印象基本一致。然而，密度分析为海马区不同亚区和层的相对AChE含量提供了客观且更精确的表达。特别是，酶含量相同的区域以及活性仅略有差异的区域能够轻松且明确地被识别。鉴于此，简要考虑了COD未来有前景的用途。
