Tano T, Lundgren D
Appl Environ Microbiol. 1978 Jun;35(6):1198-205. doi: 10.1128/aem.35.6.1198-1205.1978.
Thiobacillus ferrooxidans is an acidophilic organism important to metal leaching of low-grade ores. The aforementioned importance is related to the ability of the bacterium to oxidize reduced iron and sulfur, principally found in nature as pyrite (FeS2). The present study dealt with sulfide oxidation at low pH values and the involvement of the cell envelope in the process of the inorganic oxidations. Sulfide oxidation was noted in spheroplasts of T. ferrooxidans prepared by enzymatic and chemical treatments and partially purified by differential centrifugation. No enzyme activities were noted in membrane fractions containing enrichments of lipopolysaccharide symbolic of outer membrane material or in membrane vesicles containing (or associated with) higher levels of proteins. Results to date indicate that in an acid milieu the envelope structure containing both the outer membrane and the intact inner cytoplasmic membrane is required for sulfide oxidation.
氧化亚铁硫杆菌是一种嗜酸生物，对低品位矿石的金属浸出很重要。上述重要性与该细菌氧化还原态铁和硫的能力有关，还原态铁和硫在自然界中主要以黄铁矿（FeS₂）的形式存在。本研究涉及低pH值下的硫化物氧化以及细胞包膜在无机氧化过程中的作用。在通过酶法和化学处理制备并通过差速离心部分纯化的氧化亚铁硫杆菌原生质球中观察到了硫化物氧化。在富含外膜物质标志性脂多糖的膜组分中或在含有（或与）较高水平蛋白质相关的膜泡中未观察到酶活性。迄今为止的结果表明，在酸性环境中，硫化物氧化需要包含外膜和完整内膜的包膜结构。
