Legesse Fisseha Bekele, Meyer Tobias, Heuke Sandro, Gottschall Thomas, Pascher Torbjörn, Limpert Jens, Tünnermann Andreas, Schmitt Michael, Popp Jürgen
Opt Lett. 2017 Jan 15;42(2):183-186. doi: 10.1364/OL.42.000183.
We have developed a dual-focus coherent anti-Stokes Raman scattering (CARS) microscope based on a dual output, compact fiber laser source. The underlying concepts of time-multiplexed, two-beam scanning and demultiplexed detection that we already employed for second-harmonic generation are here naturally extended for CARS microscopy. The layout of a robust, all-fiber laser source was reconfigured to provide two outputs, each containing the two colors necessary for the CARS process. The utilization of the design for simultaneously imaging two laterally or axially separated fields of view and, thus, inherently speeding up the image acquisition process, is demonstrated on human artery tissue samples.
我们基于双输出紧凑型光纤激光源开发了一种双焦点相干反斯托克斯拉曼散射(CARS)显微镜。我们已用于二次谐波产生的时分复用、双光束扫描和解复用检测的基本概念在此自然地扩展到了CARS显微镜。对一个坚固的全光纤激光源的布局进行了重新配置,以提供两个输出,每个输出都包含CARS过程所需的两种颜色。在人体动脉组织样本上展示了利用该设计同时对两个横向或轴向分离的视野进行成像,从而固有地加快图像采集过程的情况。