Kavitha Nowroji, Chen Yeng, Kanwar Jagat R, Sasidharan Sreenivasan
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, USM 11800, Pulau Pinang, Malaysia.
Dental Research & Training Unit, and Oral Cancer Research and Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Biomed Pharmacother. 2017 Mar;87:609-620. doi: 10.1016/j.biopha.2016.12.127. Epub 2017 Jan 9.
Phaleria macrocarpa (Boerl.) is a well-known medicinal plant and have been extensively used as traditional medicine for ages in treatment of various diseases. The purpose of this study was to determine the in situ cytotoxicity effect P. macrocarpa fruit ethyl acetate fraction (PMEAF) by using various conventional and modern microscopy techniques. The cytotoxicity of PMEAF treated MDA-MB-231 cells was determined through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay and CyQuant Cell Proliferation Assay after 24h of treatment. Both results were indicated that the PMEAF is a potential anticancer agent with the average IC values of 18.10μg/mL by inhibiting the MDA-MB-231 cell proliferation. Various conventional and modern microscopy techniques such as light microscopy, holographic microscopy, transmission (TEM) and scanning (SEM) electron microscope were used for the observation of morphological changes in PMEAF treated MDA-MB-231cells for 24h. The characteristic of apoptotic cell death includes cell shrinkage, membrane blebs, chromatin condensation and the formation of apoptotic bodies were observed. PMEAF might be the best candidate for developing more potent anticancer drugs or chemo-preventive supplements.
大果木鳖（Phaleria macrocarpa (Boerl.)）是一种著名的药用植物，长期以来一直被广泛用作治疗各种疾病的传统药物。本研究的目的是通过使用各种传统和现代显微镜技术来确定大果木鳖果实乙酸乙酯馏分（PMEAF）的原位细胞毒性作用。在处理24小时后，通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）细胞毒性试验和CyQuant细胞增殖试验来测定PMEAF处理的MDA-MB-231细胞的细胞毒性。两个结果均表明PMEAF是一种潜在的抗癌剂，通过抑制MDA-MB-231细胞增殖，其平均IC值为18.10μg/mL。使用各种传统和现代显微镜技术，如光学显微镜、全息显微镜、透射电子显微镜（TEM）和扫描电子显微镜（SEM），来观察PMEAF处理24小时的MDA-MB-231细胞的形态变化。观察到凋亡细胞死亡的特征包括细胞收缩、膜泡形成、染色质浓缩和凋亡小体的形成。PMEAF可能是开发更有效的抗癌药物或化学预防补充剂的最佳候选物。
