Hyodoh Hideki, Matoba Kotaro, Murakami Manabu, Matoba Tomoko, Saito Atsuko, Feng Fei, Jin Shigeki
Center for Cause of Death Investigation, Graduate School of Medicine, Hokkaido University, Japan.
Center for Cause of Death Investigation, Graduate School of Medicine, Hokkaido University, Japan; Department of Forensic Medicine, Graduate School of Medicine, Hokkaido University, Japan.
Leg Med (Tokyo). 2017 Jan;24:19-23. doi: 10.1016/j.legalmed.2016.10.009. Epub 2016 Oct 25.
To evaluate the usefulness of freezing preparation for macroscopic investigation in advanced putrefied brain.
After sealing in individual plastic bags, 10 pig heads were stored at 20°C for 5days allow postmortem change (putrefaction) to progress. After an observation period, they were divided into 2 groups to evaluate the usefulness of the freezing effect in macroscopic investigation. The process over the postmortem period and the freezing process were examined.
At day-5, the presence of air density was detected between the inner surface of the cranium and the brain parenchyma. Intra-cranial air accumulation presented on CT in all heads. In the control group, the brain parenchyma leaked out from the hole in the meninges, and the gray-white matter difference was clear in 3/72 (4.2%), moderate in 7/72 (9.7%), ambiguous in 17/72 (23.6%), and poor in 45/72 (62.5%). In the freezing group, the brain parenchyma presented homogeneous low density after more than 14h freezing. On opening the cranium, the entire brains were frozen, and the gray-white matter difference was clear in 33/72 (46.0%), moderate in 17/72 (24.0%), ambiguous in 15/72 (21.0%), and poor in 7/72 (10.0%). The freezing group afforded greater clarity in the gray-white matter inspection (p<0.05).
Freezing preparation was useful for the macroscopic investigation of putrefied brain compared with the ordinary autopsy.
评估冷冻处理在晚期腐败脑宏观检查中的作用。
将10个猪头分别装入塑料袋密封后，于20°C保存5天，以使死后变化（腐败）进展。观察期结束后，将其分为两组以评估冷冻效果在宏观检查中的作用。检查了死后阶段及冷冻过程。
在第5天，颅骨内表面与脑实质之间检测到空气密度。所有猪头的CT均显示颅内积气。在对照组中，脑实质从脑膜的孔洞中漏出，灰白质差异清晰的占3/72（4.2%），中等清晰的占7/72（9.7%），不清晰的占17/72（23.6%），差的占45/72（62.5%）。在冷冻组中，冷冻14小时以上后脑实质呈现均匀低密度。打开颅骨后，整个大脑均被冷冻，灰白质差异清晰的占33/72（46.0%），中等清晰的占17/72（24.0%），不清晰的占15/72（21.0%），差的占7/72（10.0%）。冷冻组在灰白质检查方面清晰度更高（p<0.05）。
与普通尸检相比，冷冻处理对腐败脑的宏观检查有用。
