Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Anal Chim Acta. 2017 Feb 15;954:97-104. doi: 10.1016/j.aca.2016.11.044. Epub 2016 Nov 25.
A new benzothiazole-based fluorescent probe 2-(benzo[d]thiazol-2-yl)-4-(1,3- dithian-2-yl)phenol (BT) with two different reaction sites, a thioacetal group (site 1 for Hg), and O and N atoms of the benzothiazole dye (site 2 for Cu), was designed and synthesized. The probe BT showed ratiometric fluorescent response to Hg and fluorescence quenching behavior to Cu, which induces naked-eye fluorescent color changes from green to blue and colorless, respectively. Moreover, it displayed highly sensitivity and selectivity toward Hg and Cu without interference from other metal ions. The sensing mechanisms were also confirmed by H NMR titration, mass spectrum and Job's plot analyses. Finally, probe BT was successfully used for fluorescent imaging of Hg and Cu in living cells, demonstrating its potential applications in biological science.
一种新的基于苯并噻唑的荧光探针 2-(苯并[d]噻唑-2-基)-4-(1,3-二硫戊环-2-基)苯酚（BT），具有两个不同的反应位点，一个硫缩醛基团（Hg 的位点 1）和苯并噻唑染料的 O 和 N 原子（Cu 的位点 2），被设计和合成。探针 BT 对 Hg 表现出比率型荧光响应和对 Cu 的荧光猝灭行为，分别导致肉眼可见的荧光颜色从绿色变为蓝色和无色。此外，它对 Hg 和 Cu 具有高度的灵敏度和选择性，不受其他金属离子的干扰。传感机制也通过 H NMR 滴定、质谱和 Job 图分析得到证实。最后，探针 BT 成功地用于活细胞中 Hg 和 Cu 的荧光成像，展示了其在生物科学中的潜在应用。
