Van der Auwera P, Husson M
Service de Médecine, Institut Jules Bordet, Université Libre de Bruxelles, Belgium.
J Antimicrob Chemother. 1989 Sep;24(3):347-53. doi: 10.1093/jac/24.3.347.
We have studied the influence of rifampicin and ansamycin on chemotaxis, random migration and adherence to plastic of human neutrophils. Neutrophils were preincubated for 30 min at 37 degrees C with the antibiotics. Chemotaxis was studied in antibiotic-free and antibiotic-containing agar using zymosan-activated serum and fMLP as stimuli. Adherence was studied in plastic microtitre plates. Rifampicin and LM427 (ansamycin, 6 mg/l) did not impair differential migration, but inhibited random migration. At clinically achievable concentration (greater than or equal to 6 mg/l), inhibition was less than or equal to 18%. The chemokinetic response curve induced by different concentrations of fMLP was not modified by the two antibiotics. Adherence to polystyrene was significantly increased and could explain the inhibition of random migration.
我们研究了利福平及安莎霉素对人中性粒细胞趋化性、随机迁移及对塑料黏附性的影响。将中性粒细胞与抗生素在37℃预孵育30分钟。使用酵母聚糖激活血清和fMLP作为刺激物，在不含抗生素和含抗生素的琼脂中研究趋化性。在塑料微量滴定板中研究黏附性。利福平和LM427（安莎霉素，6毫克/升）不损害差异性迁移，但抑制随机迁移。在临床可达到的浓度（大于或等于6毫克/升）下，抑制率小于或等于18%。两种抗生素未改变不同浓度fMLP诱导的化学动力学反应曲线。对聚苯乙烯的黏附性显著增加，这可以解释随机迁移受到抑制的原因。
