Son Hyo-Eun, Kim Tae Hoon, Jang Won-Gu
Department of Biotechnology, School of Engineering, Daegu University, Gyeongsan 38453, Republic of Korea; Research Institute of Anti-Aging, Daegu University, Gyeongsan 38453, Republic of Korea.
Department of Food Science and Biotechnology, Daegu University, Gyeongsan 38453, Republic of Korea.
Bioorg Med Chem Lett. 2017 Mar 1;27(5):1301-1303. doi: 10.1016/j.bmcl.2016.12.070. Epub 2016 Dec 30.
Curculactones A and B are rare γ-lactone derivatives obtained from yellow, natural curcumin following γ-irradiation, and are a type of small molecules with a moderate anti-obesity effect. However, the exact role of curculactones A and B in osteoblast differentiation is unknown. In this study, the effects of curculactones A and B on the differentiation of the mesenchymal cell line C3H10T1/2 and pre-osteoblast cell line MC3T3-E1 to osteoblasts were examined. Curculactones A or B could markedly increase the mRNA levels of osteogenic marker genes and alkaline phosphatase (ALP) activity. Collectively, our findings indicate that curculactones A or B induced osteoblast differentiation through osteogenic expression of genes such as distal-less homeobox 5 (Dlx5), runt-related transcription factor 2 (Runx2), ALP, and osteocalcin (OC).
姜黄内酯A和B是γ射线辐照天然黄色姜黄素后得到的罕见γ-内酯衍生物，是一类具有中等抗肥胖作用的小分子。然而，姜黄内酯A和B在成骨细胞分化中的确切作用尚不清楚。在本研究中，检测了姜黄内酯A和B对间充质细胞系C3H10T1/2和前成骨细胞系MC3T3-E1向成骨细胞分化的影响。姜黄内酯A或B可显著提高成骨标记基因的mRNA水平和碱性磷酸酶（ALP）活性。总的来说，我们的研究结果表明，姜黄内酯A或B通过远端同源盒5（Dlx5）、 runt相关转录因子2（Runx2）、ALP和骨钙素（OC）等基因的成骨表达诱导成骨细胞分化。
