Denis Maghil, Ramasamy Sivakumar Mullaivanam, Kamalanathan Tamilarasan, Thayappan Karthigayani, Mannarreddy Prabu, Doss Bhuvaneswari Selvaputhiran, Munusamy Arumugam
Laboratory of Pathobiology, Department of Zoology, University of Madras, Chennai, Tamil Nadu 600025, India.
Laboratory of Pathobiology, Department of Zoology, University of Madras, Chennai, Tamil Nadu 600025, India.
Int J Biol Macromol. 2017 Apr;97:258-263. doi: 10.1016/j.ijbiomac.2017.01.026. Epub 2017 Jan 9.
The lectin, Pjlec isolated from the hemolymph of the freshwater crab Paratelphusa jacquemontii hemagglutinated (HA) with mice, rabbit and rat erythrocytes. However, the lectin failed to agglutinate neraminidase treated asialylated erythrocytes showing its sialic acid specificity. The poyacyrlamide gel electrophoresis of lectin yielded 310kDa proteins, on sodium sulphate dodecyl (SDS) gel appeared as a tetramer with subunits of 76kDa. The observation of in vitro phagocytosis in granular hemocytes of lectin opsonized rabbit erythrocyte by Transmission electron microscopy (TEM) showed the release of lytic vesicles by exocytosis prior to engulfment. The Pjlec lectin also showed an ability to oxidize L-3, 4 dihydroxyphenylalanine (L-DOPA) and in hemocyte lysate preparation (HLS) was enhanced on reduction with SDS and on proteolytic cleavage with trypsin. The lectin appeared to have a regulatory role in activation of enzyme activity associated with phagocytosis and melanin formation.
从淡水蟹Paratelphusa jacquemontii血淋巴中分离出的凝集素Pjlec能使小鼠、兔和大鼠的红细胞发生血细胞凝集（HA）。然而，该凝集素不能凝集经神经氨酸酶处理的去唾液酸红细胞，显示出其对唾液酸的特异性。凝集素的聚丙烯酰胺凝胶电泳产生了310kDa的蛋白质，在十二烷基硫酸钠（SDS）凝胶上呈现为具有76kDa亚基的四聚体。通过透射电子显微镜（TEM）观察凝集素调理的兔红细胞在颗粒血细胞中的体外吞噬作用，结果显示在吞噬之前通过胞吐作用释放了裂解小泡。Pjlec凝集素还表现出氧化L-3,4-二羟基苯丙氨酸（L-DOPA）的能力，并且在血细胞裂解液制备（HLS）中，用SDS还原和用胰蛋白酶进行蛋白水解切割后其活性增强。该凝集素似乎在与吞噬作用和黑色素形成相关的酶活性激活中具有调节作用。
