Vanderkop P A, MacNeil J D
Toxicology Research Group, University of Saskatchewan, Saskatoon, Canada.
J Assoc Off Anal Chem. 1989 Sep-Oct;72(5):735-8.
Monensin is an effective anticoccidial agent widely used in the poultry industry. Because of concerns over its toxicity, a sensitive, reliable, fast, and simple method for residue detection in poultry tissues is desirable from both a diagnostic and a regulatory view. Many methods of detection are excluded due to this ionophore's complex chemical and biological nature. A common method used for its detection is thin-layer chromatography/bioautography (TLC/B), although this is usually only semiquantitative. A new TLC/B method for monensin detection in poultry tissues was developed and is reported here. Recovery from cardiac muscle, skeletal muscle, and liver and kidney tissues is in the range of 93-97%. A detection limit of 250 micrograms/kg with 99% reliability was achieved with this method. Lower limits (to 10 micrograms/kg) were detectable, but with lower reliability (60%). Quantitative analysis is not possible on samples from fatty tissue.
莫能菌素是一种有效的抗球虫剂，广泛应用于家禽业。由于担心其毒性，从诊断和监管角度来看，都需要一种灵敏、可靠、快速且简单的家禽组织中残留检测方法。由于这种离子载体复杂的化学和生物学性质，许多检测方法都被排除在外。用于其检测的常用方法是薄层色谱/生物自显影法（TLC/B），不过这通常只是半定量的。本文报道了一种用于检测家禽组织中莫能菌素的新TLC/B方法。从心肌、骨骼肌、肝脏和肾脏组织中的回收率在93%至97%之间。该方法实现了250微克/千克的检测限，可靠性为99%。下限（低至10微克/千克）也可检测到，但可靠性较低（60%）。对脂肪组织样本无法进行定量分析。
